Cloned (Comment) | Organism |
---|---|
expression of the His-tagged inactive pro-enzyme in Escherichia coli BL21(DE3) | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Entamoeba histolytica | |
iodoacetamide | - |
Entamoeba histolytica | |
leupeptin | - |
Entamoeba histolytica | |
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane | ie.e. E-64, irreversible | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Entamoeba histolytica | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | processing of the inactive pro-enzyme to the mature active form by treatment with 10 mM DTT and 0.04% SDS at 37°C and pH 8.8, SDS can be substituted by Triton X-100 and octylglucoside but these are 3fold less efficient | Entamoeba histolytica |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged inactive pro-enzyme to homogeneity | Entamoeba histolytica |
Renatured (Comment) | Organism |
---|---|
renaturation of purified inactive pro-enzyme by a 100fold dilution in a buffer containing reduced and oxidized thiols leading to a soluble inactive pro-enzyme | Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
170 | - |
purified native enzyme, substrate azocasein | Entamoeba histolytica |
180 | - |
purified recombinant enzyme, substrate azocasein | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Entamoeba histolytica | fragments of azocasein | - |
? | |
benzyloxycarbonyl-Ala-Arg-Arg-4-nitroanilide + H2O | - |
Entamoeba histolytica | benzyloxycarbonyl-Ala-Arg-Arg + 4-nitroaniline | - |
? | |
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O | - |
Entamoeba histolytica | benzyloxycarbonyl-Arg-Arg + 4-nitroaniline | - |
? | |
Bovine serum albumin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
Collagen + H2O | - |
Entamoeba histolytica | ? | - |
? | |
Fibronectin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
Gelatin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
Hemoglobin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
immunoglobulin G + H2O | - |
Entamoeba histolytica | ? | - |
? | |
additional information | no activity with benzyloxycarbonyl-Phe-Arg-4-nitroanilide | Entamoeba histolytica | ? | - |
? | |
mucin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
protein + H2O | - |
Entamoeba histolytica | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000-29000, recombinant and native enzyme, reducing and nonreducing SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
EhCP5 | - |
Entamoeba histolytica |
Entamoeba histolytica cysteine proteinase 5 | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | recombinant enzyme | Entamoeba histolytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | recombinant enzyme | Entamoeba histolytica |