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Literature summary for 3.4.22.40 extracted from

  • Nishimura, C.; Tanaka, N.; Suzuki, H.; Tanaka, N.
    Purification of bleomycin hydrolase with a monoclonal antibody and its characterization (1987), Biochemistry, 26, 1574-1578.
    View publication on PubMed
Search for more literature for 3.4.22.40

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
1-(L-trans-epoxysuccinylleucylamino)-4-guanidinobutane
-
 Oryctolagus cuniculus
Cu2+
-
 Oryctolagus cuniculus
Fe2+
-
 Oryctolagus cuniculus
leupeptin
-
 Oryctolagus cuniculus
N-ethylmaleimide
-
 Oryctolagus cuniculus
p-tosyl-L-lysine chloromethyl ketone
-
 Oryctolagus cuniculus
Zn2+
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.79
-
 bleomycin B2
-
 Oryctolagus cuniculus
0.79
-
 peplomycin
-
 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 6 * 48000, SDS-PAGE Oryctolagus cuniculus
300000
-
 gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bleomycin + H2O bleomycin B2 Oryctolagus cuniculus deamido-bleomycin
-
 ?
bleomycin B2 + H2O
-
 Oryctolagus cuniculus ?
-
 ?
peplomycin + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 48000, SDS-PAGE Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.8
-
 Oryctolagus cuniculus