Any feedback?
Literature summary for 3.4.22.40 extracted from
- Bleomycin hydrolase (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1197-1201.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | binding to nucleic acids, preferably single-stranded DNA or RNA, is required for enzyme activity | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination | Rattus norvegicus |
| DNA sequence determination | Oryctolagus cuniculus |
| DNA sequence determination and analysis, chromosome localization at 19q11.2 | Homo sapiens |
| gene YCP1 or BLH1, DNA sequence determination | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutant pre3-2 is inactive with substrate Z-Leu-Leu-Glu-NH-4-nitroanilide, but can be complemented b ythe wild-type gene | Saccharomyces cerevisiae |
| V443I | the naturally occurring polymorphism of the gene encoding the enzyme is associated to sporadic Alzheimer's disease and to the amyloid precursor protein | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxymeruribenzoate | - |
Homo sapiens |  |
| 4-hydroxymeruribenzoate | - |
Oryctolagus cuniculus | |
| 4-hydroxymeruribenzoate | - |
Rattus norvegicus | |
| 4-hydroxymeruribenzoate | - |
Streptomyces verticillus | |
| E64 | - |
Homo sapiens | |
| E64 | - |
Oryctolagus cuniculus | |
| E64 | - |
Rattus norvegicus | |
| E64 | - |
Streptomyces verticillus | |
| iodoacetate | - |
Homo sapiens | |
| iodoacetate | - |
Oryctolagus cuniculus | |
| iodoacetate | - |
Rattus norvegicus | |
| iodoacetate | - |
Streptomyces verticillus | |
| leupeptin | weak inhibition | Rattus norvegicus | |
| additional information | no inhibition by leupeptin | Homo sapiens | |
| additional information | no inhibition by cystatin A and cystatin C | Rattus norvegicus | |
| additional information | no inhibition by leupeptin | Streptomyces verticillus | |
| NEM | - |
Homo sapiens | |
| NEM | - |
Oryctolagus cuniculus | |
| NEM | - |
Rattus norvegicus | |
| NEM | - |
Streptomyces verticillus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | a self-compartmentalizing enzyme | Homo sapiens | - |
- |
| additional information | a self-compartmentalizing enzyme | Rattus norvegicus | - |
- |
| additional information | a self-compartmentalizing enzyme | Oryctolagus cuniculus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | glycometalloenzyme | Streptomyces verticillus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 300000 | - |
about | Homo sapiens |
| 300000 | - |
about | Rattus norvegicus |
| 300000 | - |
about | Oryctolagus cuniculus |
| 300000 | - |
about | Streptomyces verticillus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bleomycin + H2O | Rattus norvegicus | detoxification reaction | ? | - |
? | |
| bleomycin + H2O | Oryctolagus cuniculus | detoxification reaction | ? | - |
? | |
| bleomycin + H2O | Homo sapiens | detoxification reaction, high enzyme expression level leads to resistance against the drug | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | the enzyme expression is regulated by galactose and Gal4p,expression pattrern | ? | - |
? | |
| Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O | Saccharomyces cerevisiae | no activity in vitro due to requirement of binding to DNA-recognition sites of ztranscriptional activator Gal4p | Z-Leu-Leu-Glu + 4-nitroaniline | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
gene YCP1 or BLH1 | - |
| Streptomyces verticillus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glycometalloenzyme | Streptomyces verticillus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| Burkitt's lymphoma cell | high expression level | Homo sapiens | - |
| leukemia cell | high expression level | Homo sapiens | - |
| additional information | no expression in Hodgkin's lymphoma cells | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bleomycin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| bleomycin + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| bleomycin + H2O | detoxification reaction | Rattus norvegicus | ? | - |
? | |
| bleomycin + H2O | detoxification reaction | Oryctolagus cuniculus | ? | - |
? | |
| bleomycin + H2O | detoxification reaction, high enzyme expression level leads to resistance against the drug | Homo sapiens | ? | - |
? | |
| bleomycin A2 + H2O | preferred to bleomycin B | Homo sapiens | ? | - |
? | |
| bleomycin B2 + H2O | - |
Homo sapiens | ? | - |
? | |
| additional information | the enzyme expression is regulated by galactose and Gal4p,expression pattrern | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview | Homo sapiens | ? | - |
? | |
| additional information | the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview | Rattus norvegicus | ? | - |
? | |
| additional information | the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview | Oryctolagus cuniculus | ? | - |
? | |
| Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O | no activity in vitro due to requirement of binding to DNA-recognition sites of ztranscriptional activator Gal4p | Saccharomyces cerevisiae | Z-Leu-Leu-Glu + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | homohexamer, three-dimensional structure | Homo sapiens |
| hexamer | homohexamer, three-dimensional structure | Rattus norvegicus |
| hexamer | homohexamer, three-dimensional structure | Oryctolagus cuniculus |
| hexamer | homohexamer, three-dimensional structure | Streptomyces verticillus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BH | - |
Homo sapiens |
| BH | - |
Rattus norvegicus |
| BH | - |
Saccharomyces cerevisiae |
| BH | - |
Oryctolagus cuniculus |
| BH | - |
Streptomyces verticillus |
| Gal6p | - |
Saccharomyces cerevisiae |
| More | the enzyme belongs to the C1B peptidase family | Homo sapiens |
| More | the enzyme belongs to the C1B peptidase family | Rattus norvegicus |
| More | the enzyme belongs to the C1B peptidase family | Saccharomyces cerevisiae |
| More | the enzyme belongs to the C1B peptidase family | Oryctolagus cuniculus |
| yeast cysteine protease | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Streptomyces verticillus |
| 7 | 7.5 | - |
Oryctolagus cuniculus |
| 7.2 | - |
- |
Homo sapiens |
| 7.5 | - |
- |
Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
