Cloned (Comment) | Organism |
---|---|
cDNA cloned from primary HSCs using RACE-PCR | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | downregulation of cathepsin F mRNA by E-64d; treatment with retinol/palmitic acid increases mRNA-level of cystatin B coupled with decrease in cathepsin F activity; treatment with retinol/palmitic acid increases mRNA-level of cystatin C coupled with decrease in cathepsin F activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | Western blot | Rattus norvegicus | 5829 | - |
nucleus | immunofluorescence microscopy, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leucin-Arginin-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm | Rattus norvegicus | 5634 | - |
nucleus | Western blot, immunofluorescence microscopy, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm | Rattus norvegicus | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q499S6 | Wistar rat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hematopoietic stem cell | - |
Rattus norvegicus | - |
HSC-2 cell | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
adding E-64d (inhibitor) results in drastically inhibition of enzyme activity in cytosol and nucleus, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm, confirmed by cathepsin F siRNA treatment | Rattus norvegicus |
additional information | - |
higher activity in nucleus than in cytosol, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm, differentiation between cathepsin F and other cathepsins by using specific inhibitors and substrates | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cathepsin f | - |
Rattus norvegicus |