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Literature summary for 3.4.22.41 extracted from

  • Sripa, J.; Laha, T.; To, J.; Brindley, P.J.; Sripa, B.; Kaewkes, S.; Dalton, J.P.; Robinson, M.W.
    Secreted cysteine proteases of the carcinogenic liver fluke, Opisthorchis viverrini: regulation of cathepsin F activation by autocatalysis and trans-processing by cathepsin B (2010), Cell. Microbiol., 12, 781-795.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.41

Activating Compound

Activating Compound Comment Organism Structure
additional information cathepsin F is secreted as an inactive zymogen that autocatalytically processes and activates to a mature enzyme at pH 4.5 (after 6 h incubation) via an intermolecular cleavage at the prosegment-mature domain junction, the active cathepsin B1 zymogen is capable of trans-activating cathepsin F by proteolytic removal of its prosegment at pH 5.5 Opisthorchis viverrini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 cathepsin F proenzyme in the yeast medium is present as two major protein bands migrating at 41000 Da and 47000 Da as a result of differential addition of N-linked glycans Opisthorchis viverrini
47000
-
 cathepsin F proenzyme in the yeast medium is present as two major protein bands migrating at 41000 Da and 47000 Da as a result of differential addition of N-linked glycans Opisthorchis viverrini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Hemoglobin + H2O Opisthorchis viverrini cathepsin F and cathepsin B can work in concert to hydrolyse hemoglobin at low pH ?
-
 ?
additional information Opisthorchis viverrini cathepsin F degrades extracellular matrix proteins more effectively than capthespin B1 at physiological pH ?
-
 ?

Organism

Organism UniProt Comment Textmining
Opisthorchis viverrini
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Opisthorchis viverrini

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Opisthorchis viverrini
-
egg
-
 Opisthorchis viverrini
-
metacercaria
-
 Opisthorchis viverrini
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O low activity Opisthorchis viverrini ?
-
 ?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O high activity Opisthorchis viverrini benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O high activity Opisthorchis viverrini ?
-
 ?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O very low activity Opisthorchis viverrini ?
-
 ?
Hemoglobin + H2O cathepsin F and cathepsin B can work in concert to hydrolyse hemoglobin at low pH Opisthorchis viverrini ?
-
 ?
additional information cathepsin F degrades extracellular matrix proteins more effectively than capthespin B1 at physiological pH Opisthorchis viverrini ?
-
 ?
additional information the fully activated cathepsin F cleaves fluorogenic-NHMec substrates with the preference Val-Leu-Arg>Leu-Arg>Phe-Arg>Arg-Arg with little or no activity against Pro-Arg or Pro-Lys substrates Opisthorchis viverrini ?
-
 ?
Pro-Arg-7-amido-4-methylcoumarin + H2O very low activity Opisthorchis viverrini ?
-
 ?
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O very low activity Opisthorchis viverrini ?
-
 ?
tert-butyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O highest activity Opisthorchis viverrini ?
-
 ?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O very low activity Opisthorchis viverrini ?
-
 ?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O very low activity Opisthorchis viverrini ?
-
 ?
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin + H2O very low activity Opisthorchis viverrini ?
-
 ?

Synonyms

Synonyms Comment Organism
CF-1
-
 Opisthorchis viverrini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Opisthorchis viverrini

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8.5
-
 Opisthorchis viverrini

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 cathepsin F zymogen is inactive at pH 5.5 Opisthorchis viverrini