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Literature summary for 3.4.22.41 extracted from

  • Ahn, S.J.; Kim, N.Y.; Seo, J.S.; Je, J.E.; Sung, J.H.; Lee, S.H.; Kim, M.S.; Kim, J.K.; Chung, J.K.; Lee, H.H.
    Molecular cloning, mRNA expression and enzymatic characterization of cathepsin F from olive flounder (Paralichthys olivaceus) (2009), Comp. Biochem. Physiol. B, 154, 211-220.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Brij 35 273.6% relative activity at 0.01% (v/v) Paralichthys olivaceus
Triton X-100 310.29% relative activity at 0.01% (v/v) Paralichthys olivaceus
Tween 20 282.7% relative activity at 0.01% (v/v) Paralichthys olivaceus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alphaMCR cells using the pGEX-4 T-1 expression vector system Paralichthys olivaceus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 15.28% inhibition at 0.1 mM Paralichthys olivaceus
antipain complete inhibition at 0.1 mM Paralichthys olivaceus
Aprotinin 36.94% inhibition at 0.1 mM Paralichthys olivaceus
chymostatin 35.42% inhibition at 0.1 mM Paralichthys olivaceus
Co2+ 6.85% residual activity at 5 mM Paralichthys olivaceus
Cu2+ 11.6% residual activity at 5 mM Paralichthys olivaceus
E-64 56.3% inhibition at 0.1 mM Paralichthys olivaceus
EDTA 6.52% inhibition at 0.1 mM Paralichthys olivaceus
EGTA 4.72% inhibition at 0.1 mM Paralichthys olivaceus
Hg2+ 0.81% residual activity at 5 mM Paralichthys olivaceus
leupeptin complete inhibition at 0.1 mM Paralichthys olivaceus
additional information not inhibited by pepstatin A Paralichthys olivaceus
NEM 44.59% inhibition at 0.1 mM Paralichthys olivaceus
phenylmethylsufonyl fluoride 13.22% inhibition at 0.1 mM Paralichthys olivaceus
SDS complete inhibition at 0.05% (w/v) Paralichthys olivaceus
Zn2+ 5.94% residual activity at 1 mM Paralichthys olivaceus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 149.95% relative activity at 1 mM Paralichthys olivaceus
K+ 143.7% relative activity at 1 mM Paralichthys olivaceus
Mg2+ 133.38% relative activity at 1 mM Paralichthys olivaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
recombinant His-tag fusion protein, SDS-APGE Paralichthys olivaceus

Organism

Organism UniProt Comment Textmining
Paralichthys olivaceus B2Z446 olive flounder
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Paralichthys olivaceus

Source Tissue

Source Tissue Comment Organism Textmining
brain high expression Paralichthys olivaceus
-
epidermal mucus immunostaining using anti-PoCtF antibody was strongest on the epidermal mucus in the fin Paralichthys olivaceus
-
esophagus high expression Paralichthys olivaceus
-
eye high expression Paralichthys olivaceus
-
gill
-
Paralichthys olivaceus
-
head kidney high expression Paralichthys olivaceus
-
heart high expression Paralichthys olivaceus
-
intestine
-
Paralichthys olivaceus
-
kidney high expression Paralichthys olivaceus
-
liver high expression Paralichthys olivaceus
-
additional information ubiquitous expression throughout the entirety of healthy tissues Paralichthys olivaceus
-
muscle
-
Paralichthys olivaceus
-
pyloric cecum
-
Paralichthys olivaceus
-
spleen high expression Paralichthys olivaceus
-
stomach
-
Paralichthys olivaceus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
Paralichthys olivaceus benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?
Gelatin + H2O purified CtF is capable of hydrolyzing 0.1% gelatin at pH 7.5 Paralichthys olivaceus ?
-
?
additional information the enzyme is not able to hydrolyze protein substrates such as azocasein, fibrinogen, and bovine serum albumin Paralichthys olivaceus ?
-
?

Synonyms

Synonyms Comment Organism
CtF
-
Paralichthys olivaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Paralichthys olivaceus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5
-
Paralichthys olivaceus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
activity is greatly decreased at pH 5 (lysosomal pH) and below Paralichthys olivaceus

Expression

Organism Comment Expression
Paralichthys olivaceus CtF expressions increases significantly in gill at 3 h post-injection with lipopolysaccharide up