Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij 35 | 273.6% relative activity at 0.01% (v/v) | Paralichthys olivaceus | |
Triton X-100 | 310.29% relative activity at 0.01% (v/v) | Paralichthys olivaceus | |
Tween 20 | 282.7% relative activity at 0.01% (v/v) | Paralichthys olivaceus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alphaMCR cells using the pGEX-4 T-1 expression vector system | Paralichthys olivaceus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 15.28% inhibition at 0.1 mM | Paralichthys olivaceus | |
antipain | complete inhibition at 0.1 mM | Paralichthys olivaceus | |
Aprotinin | 36.94% inhibition at 0.1 mM | Paralichthys olivaceus | |
chymostatin | 35.42% inhibition at 0.1 mM | Paralichthys olivaceus | |
Co2+ | 6.85% residual activity at 5 mM | Paralichthys olivaceus | |
Cu2+ | 11.6% residual activity at 5 mM | Paralichthys olivaceus | |
E-64 | 56.3% inhibition at 0.1 mM | Paralichthys olivaceus | |
EDTA | 6.52% inhibition at 0.1 mM | Paralichthys olivaceus | |
EGTA | 4.72% inhibition at 0.1 mM | Paralichthys olivaceus | |
Hg2+ | 0.81% residual activity at 5 mM | Paralichthys olivaceus | |
leupeptin | complete inhibition at 0.1 mM | Paralichthys olivaceus | |
additional information | not inhibited by pepstatin A | Paralichthys olivaceus | |
NEM | 44.59% inhibition at 0.1 mM | Paralichthys olivaceus | |
phenylmethylsufonyl fluoride | 13.22% inhibition at 0.1 mM | Paralichthys olivaceus | |
SDS | complete inhibition at 0.05% (w/v) | Paralichthys olivaceus | |
Zn2+ | 5.94% residual activity at 1 mM | Paralichthys olivaceus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 149.95% relative activity at 1 mM | Paralichthys olivaceus | |
K+ | 143.7% relative activity at 1 mM | Paralichthys olivaceus | |
Mg2+ | 133.38% relative activity at 1 mM | Paralichthys olivaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
recombinant His-tag fusion protein, SDS-APGE | Paralichthys olivaceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paralichthys olivaceus | B2Z446 | olive flounder | - |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Paralichthys olivaceus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | high expression | Paralichthys olivaceus | - |
epidermal mucus | immunostaining using anti-PoCtF antibody was strongest on the epidermal mucus in the fin | Paralichthys olivaceus | - |
esophagus | high expression | Paralichthys olivaceus | - |
eye | high expression | Paralichthys olivaceus | - |
gill | - |
Paralichthys olivaceus | - |
head kidney | high expression | Paralichthys olivaceus | - |
heart | high expression | Paralichthys olivaceus | - |
intestine | - |
Paralichthys olivaceus | - |
kidney | high expression | Paralichthys olivaceus | - |
liver | high expression | Paralichthys olivaceus | - |
additional information | ubiquitous expression throughout the entirety of healthy tissues | Paralichthys olivaceus | - |
muscle | - |
Paralichthys olivaceus | - |
pyloric cecum | - |
Paralichthys olivaceus | - |
spleen | high expression | Paralichthys olivaceus | - |
stomach | - |
Paralichthys olivaceus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Paralichthys olivaceus | benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
Gelatin + H2O | purified CtF is capable of hydrolyzing 0.1% gelatin at pH 7.5 | Paralichthys olivaceus | ? | - |
? | |
additional information | the enzyme is not able to hydrolyze protein substrates such as azocasein, fibrinogen, and bovine serum albumin | Paralichthys olivaceus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CtF | - |
Paralichthys olivaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Paralichthys olivaceus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Paralichthys olivaceus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
activity is greatly decreased at pH 5 (lysosomal pH) and below | Paralichthys olivaceus |
Organism | Comment | Expression |
---|---|---|
Paralichthys olivaceus | CtF expressions increases significantly in gill at 3 h post-injection with lipopolysaccharide | up |