Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Porphyromonas gingivalis | |
cysteine | most effective reducing agent for activation, maximal activation at 50 mM | Porphyromonas gingivalis | |
dithiothreitol | - |
Porphyromonas gingivalis | |
glutathione | - |
Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Gly-Gly | 200 mM, 50% inhibition | Porphyromonas gingivalis | |
iodoacetamide | 10 mM, complete inhibition | Porphyromonas gingivalis | |
N-alpha-p-tosyl-L-lysine chloromethyl ketone | 0.1 mM, complete inhibition | Porphyromonas gingivalis | |
N-ethylmaleimide | 10 mM, complete inhibition | Porphyromonas gingivalis | |
Phe-Pro-Arg-chloromethyl ketone | 0.1 mM, 95% inhibition | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | pH 8.5, 37°C | Porphyromonas gingivalis | |
0.126 | - |
D-Val-Phe-Lys-p-nitroanilide | pH 8.5, 37°C | Porphyromonas gingivalis | |
0.18 | - |
benzyloxycarbonyl-L-Lys-p-nitroanilide | pH 8.5, 37°C | Porphyromonas gingivalis | |
0.2 | - |
D-Val-Leu-Lys-p-nitroanilide | pH 8.5, 37°C | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Porphyromonas gingivalis |
105000 | - |
gel filtration without boiling | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Porphyromonas gingivalis H66 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitate, Sephadex G-150, arginine-Sepharose | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acid-soluble human placental type I collagen + H2O | - |
Porphyromonas gingivalis | fragments of acid-soluble human placental type I collagen | - |
? | |
adrenocorticotropic hormone fragment 11-24 + H2O | - |
Porphyromonas gingivalis | fragments of adrenocorticotropic hormone fragment 11-24 | - |
? | |
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis | benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O | - |
? | |
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis H66 | benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O | - |
? | |
beta-endorphin + H2O | - |
Porphyromonas gingivalis | fragments of beta-endorphin | - |
? | |
bovine hemoglobin + H2O | - |
Porphyromonas gingivalis | fragments of bovine hemoglobin | - |
? | |
bovine serum albumin + H2O | - |
Porphyromonas gingivalis | fragments of bovine serum albumin | - |
? | |
casein + H2O | - |
Porphyromonas gingivalis | fragments of casein | - |
? | |
D-Val-Leu-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis | D-Val-Leu-Lys + p-nitroaniline | - |
? | |
D-Val-Leu-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis H66 | D-Val-Leu-Lys + p-nitroaniline | - |
? | |
D-Val-Phe-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis | D-Val-Phe-Lys + p-nitroaniline | - |
? | |
D-Val-Phe-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis H66 | D-Val-Phe-Lys + p-nitroaniline | - |
? | |
human IgA + H2O | - |
Porphyromonas gingivalis | cleaved human IgA | - |
? | |
human IgG + H2O | - |
Porphyromonas gingivalis | fragments of human IgG | - |
? | |
melittin + H2O | - |
Porphyromonas gingivalis | fragments of melittin | - |
? | |
Met-Lys-bradykinin + H2O | - |
Porphyromonas gingivalis | fragments of Met-Lys-bradykinin | - |
? | |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | gingipain K cleaves specifically on the C-terminal side of peptide substrates | Porphyromonas gingivalis | N-p-tosyl-Gly-Pro-Lys + p-nitroaniline | - |
? | |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | gingipain K cleaves specifically on the C-terminal side of peptide substrates | Porphyromonas gingivalis H66 | N-p-tosyl-Gly-Pro-Lys + p-nitroaniline | - |
? | |
neurotensin + H2O | - |
Porphyromonas gingivalis | pGlu-Leu-Tyr-Glu-Asn-Lys + Pro-Arg-Arg-Pro-Tyr-Ile-Leu | - |
? | |
neurotensin + H2O | - |
Porphyromonas gingivalis H66 | pGlu-Leu-Tyr-Glu-Asn-Lys + Pro-Arg-Arg-Pro-Tyr-Ile-Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Porphyromonas gingivalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
no loss of activity | Porphyromonas gingivalis |
37 | - |
no loss of activity | Porphyromonas gingivalis |
60 | - |
rapid inactivation | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
hydrolysis of small synthetic peptides | Porphyromonas gingivalis |
8.5 | - |
with protein substrates like azocasein | Porphyromonas gingivalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | stable for several hours in the absence of cysteine, rapid loss of activity below pH 8.0 in the presence of cysteine | Porphyromonas gingivalis |