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Literature summary for 3.4.22.53 extracted from

  • Wang, J.H.; Jiang, S.T.
    Properties of calpain II from Tilapia muscle (1991), Agric. Biol. Chem., 55, 339-345.
No PubMed abstract available
Search for more literature for 3.4.22.53

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
Co2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
Cu2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
Fe2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
Hg2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
K+ inhibits at high concentrations Tilapia sp.
Na+ inhibits at high concentrations Tilapia sp.
NEM 3.0 mM, high inhibition of activity in presence or absence of Ca2+ Tilapia sp.
Ni2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
Polyethylene glycol PEG-4000, PEG-100000 or PEG-20000, inhibition at concentrations higher than 0.5% Tilapia sp.
Zn2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM 85.4% of the activation with 5 mM Ca2+ Tilapia sp.
Ca2+ activates Tilapia sp.
Mn2+ 5 mM 73.6% of the activation with 5 mM Ca2+. Synergistic activating effect with Ca2+ Tilapia sp.
Sr2+ 5 mM 91% of the activation with 5 mM Ca2+ Tilapia sp.

Organism

Organism UniProt Comment Textmining
Tilapia sp.
-
 Tilapia nilotica * Tilapia aurea
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle post-mortem Tilapia sp.
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Tilapia sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 45 10°C: about 60% of maximal activity, 45°C: about 35% of maximal activity Tilapia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Tilapia sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 50% of maximal activity, pH 8.5: about 30% of maximal activity Tilapia sp.