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Literature summary for 3.4.22.53 extracted from

  • Wang, J.H.; Ma, W.C.; Su, J.C.; Chen, C.S.; Jiang, S.T.
    Comparison of the properties of m-calpain from Tilapia and grass shrimp muscles (1993), J. Agric. Food Chem., 41, 1379-1384.
    View publication on PubMed
Search for more literature for 3.4.22.53

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, activates Penaeus monodon
2-mercaptoethanol 1 mM, activates Tilapia sp.
dithiothreitol 1 mM, activates Penaeus monodon
dithiothreitol 1 mM, activates Tilapia sp.
glutathione 1 mM, activates Penaeus monodon
glutathione 1 mM, activates Tilapia sp.

Inhibitors

Inhibitors Comment Organism Structure
antipain 0.02 mM, 82% loss of activity Penaeus monodon
antipain 0.02 mM, 87% loss of activity Tilapia sp.
calpain inhibitor I 0.002 mM, 95% loss of activity Penaeus monodon
calpain inhibitor I 0.002 mM, 90% loss of activity Tilapia sp.
calpain inhibitor II 0.002 mM, 67% loss of activity Penaeus monodon
calpain inhibitor II 0.002 mM, 93% loss of activity Tilapia sp.
Cd2+ 5 mM Penaeus monodon
Cd2+ 5 mM Tilapia sp.
Co2+ 5 mM Penaeus monodon
Cu2+ 5 mM Penaeus monodon
Cu2+ 5 mM Tilapia sp.
Fe2+ 5 mM Penaeus monodon
Fe2+ 5 mM Tilapia sp.
Fe3+ 5 mM Penaeus monodon
Fe3+ 5 mM Tilapia sp.
Hg2+ 5 mM Penaeus monodon
Hg2+ 5 mM Tilapia sp.
iodoacetic acid 1 mM, complete loss of activity Penaeus monodon
iodoacetic acid 1 mM, 99% loss of activity Tilapia sp.
K+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
leupeptin 0.002 mM, complete loss of activity Penaeus monodon
leupeptin 0.002 mM, 93% loss of activity Tilapia sp.
Mg2+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
Na+ 5 mM, inhibits in presence of 5 mM Ca2+ Tilapia sp.
Ni2+ 5 mM Penaeus monodon
Ni2+ 5 mM Tilapia sp.
PCMB 1 mM, 56% inhibition Penaeus monodon
PCMB 1 mM, 42% inhibition Tilapia sp.
Zn2+ 5 mM Penaeus monodon
Zn2+ 5 mM Tilapia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM, activates Penaeus monodon
Ba2+ 5 mM, activates Tilapia sp.
Ca2+ half-maximal activity at 2.4 mM, maximal activity at 5 mM Penaeus monodon
Ca2+ half-maximal activity at 0.23 mM, maximal activity at 1-2.5 mM Tilapia sp.
Co2+ 5 mM, activates Tilapia sp.
K+ 5 mM, activates in presence of 5 mM Ca2+ Penaeus monodon
Mg2+ 5 mM, activates in presence of 5 mM Ca2+ Penaeus monodon
Mn2+ 5 mM, activates Penaeus monodon
Mn2+ 5 mM, activates Tilapia sp.
Na+ 5 mM, activates in presence of 5 mM Ca2+ Penaeus monodon
Sr2+ 5 mM, activates Penaeus monodon
Sr2+ 5 mM, activates Tilapia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 1 * 80000 + 1 * 28000, SDS-PAGE Tilapia sp.
80000
-
 2 * 80000, SDS-PAGE Penaeus monodon
80000
-
 1 * 80000 + 1 * 28000, SDS-PAGE Tilapia sp.
110000
-
 gel filtration Tilapia sp.
160000
-
 gel filtration Penaeus monodon

Organism

Organism UniProt Comment Textmining
Penaeus monodon
-
-
-
Tilapia sp.
-
 Tilapia nilotica * Tilapia aurea
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Penaeus monodon
-
muscle
-
 Tilapia sp.
-

Storage Stability

Storage Stability Organism
-20°C, stable for 8 days, 70% inactivation after 2 months Penaeus monodon
-20°C, stable for 8 days, complete inactivation after 2 months Tilapia sp.

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Penaeus monodon
dimer 1 * 80000 + 1 * 28000, SDS-PAGE Tilapia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Penaeus monodon
30
-
-
 Tilapia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48.5
-
 10 min, 50% loss of activity Penaeus monodon
61
-
 10 min, 50% loss of activity Tilapia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Penaeus monodon
7.5
-
-
 Tilapia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 25°C, 30 min, stable in presence of 20 mM 2-mercaptoethanol Tilapia sp.
5.5
-
 25°C, 30 min, about 30% loss of activity Penaeus monodon
6.5 7.5 25°C, 30 min, stable in presence of 6 mM 2-mercaptoethanol Penaeus monodon
8.5
-
 25°C, 30 min, about 50% loss of activity Penaeus monodon