Literature summary for 3.4.22.53 extracted from

Ladrat, C.; Verrez-Bagnis, V.; Noel, J.; Fleurence, J.
Milli-calpain from sea bass (Dicentrarchus labrax) white muscle: purification, characterization of its activity and activation in vitro (2002), Mar. Biotechnol., 4, 51-62.

Search for more literature for 3.4.22.53

Inhibitors

Inhibitors	Comment	Organism
(2S,3S)-trans-epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester	0.01 mM, 50% inhibition	Dicentrarchus labrax
3,4-dichloroisocoumarin	0.05 mM, 11% inhibition	Dicentrarchus labrax
4-[[(3,4-dinitrophenyl)carbonyl]amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid	5 mM, 23% inhibition	Dicentrarchus labrax
alpha2-Macroglobulin	0.05 mg/ml, 11% inhibition	Dicentrarchus labrax
antipain	0.05 mM, complete inhibition	Dicentrarchus labrax
calpain inhibitor I	0.05 mM, 99% inhibition	Dicentrarchus labrax
calpain inhibitor II	0.05 mM, complete inhibition	Dicentrarchus labrax
Co2+	2.5 mM, strong	Dicentrarchus labrax
E-64	0.01 mM, 98% inhibition	Dicentrarchus labrax
EDTA	5 mM, complete inhibition	Dicentrarchus labrax
Fe2+	2.5 mM, strong	Dicentrarchus labrax
Fe3+	2.5 mM, complete	Dicentrarchus labrax
Hg2+	2.5 mM, complete	Dicentrarchus labrax
iodoacetic acid	0.1 mM, 99% inhibition	Dicentrarchus labrax
leupeptin	0.05 mM, 99% inhibition	Dicentrarchus labrax
Mn2+	2.5 mM, strong	Dicentrarchus labrax
N-tosyl-Lys-chloromethyl ketone	0.5 mM, 69% inhibition	Dicentrarchus labrax
N-tosyl-Phe-chloromethyl ketone	0.5 mM, 91% inhibition	Dicentrarchus labrax
NEM	5 mM, complete inhibition	Dicentrarchus labrax
Ni2+	2.5 mM, complete	Dicentrarchus labrax
PCMB	5 mM, 24% inhibition	Dicentrarchus labrax
pepstatin	0.01 mM, 99% inhibition	Dicentrarchus labrax
PMSF	1 mM, 6% inhibition	Dicentrarchus labrax
protease inhibitor	0.002 mg/ml, 6% inhibition	Dicentrarchus labrax
Zn2+	2.5 mM, complete	Dicentrarchus labrax

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.431	-	succinyl-Leu-Tyr-7-amido-4-methylcoumarin	-	Dicentrarchus labrax

Metals/Ions

Metals/Ions	Comment	Organism
Ba2+	activates	Dicentrarchus labrax
Ca2+	best activator at 2.5 mM, maximal caseinolytic activity at 2.2 mM, half-maximal caseinolytic activity at 0.312 mM	Dicentrarchus labrax
Cu2+	activates	Dicentrarchus labrax
Mn2+	activates	Dicentrarchus labrax
Sr2+	2.5 mM, 66% of the activation obtained with Ca2+, maximal caseinolytic activity at 5.9 mM, half-maximal caseinolytic activity at 1.886 mM. Autolysis in presence of 5 mM Ca2+. The 80000 Da subunit is rapidly autolyzed in two smaller bands of 73000 Da and 69000 Da. The small subunit of 24000 da is degraded into three bands of 22000 Da, 19300 Da and 17800 Da. It is not clear whether autolysis is necessary for calpain to become proteolytically active	Dicentrarchus labrax

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	1 * 80000 + 1 * 24000, SDS-PAGE	Dicentrarchus labrax
80000	-	1 * 80000 + 1 * 24000, SDS-PAGE	Dicentrarchus labrax
124000	-	gel filtration	Dicentrarchus labrax

Organism

Organism	UniProt	Comment	Textmining
Dicentrarchus labrax	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	the enzyme is neither glycosylated nor phosphorylated	Dicentrarchus labrax

Purification (Commentary)

Purification (Comment)	Organism
-	Dicentrarchus labrax

Source Tissue

Source Tissue	Comment	Organism	Textmining
white muscle	present in very low amounts, only 0.0033%	Dicentrarchus labrax	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
casein + H2O	-	Dicentrarchus labrax	?	-	?
succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O	-	Dicentrarchus labrax	?	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 80000 + 1 * 24000, SDS-PAGE	Dicentrarchus labrax

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
24	-	-	Dicentrarchus labrax

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Dicentrarchus labrax