Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star(DE3) cells | Severe acute respiratory syndrome-related coronavirus |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme N28A, hanging drop vapor diffusion method, using 1.0 M sodium malonate (pH 7.0), and 5-6% (v/v) isopropanol | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
N28A | the mutation almost completely inactivates the enzyme and results in decreased dimerization | Severe acute respiratory syndrome-related coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05642 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | wild type enzyme, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C | Severe acute respiratory syndrome-related coronavirus | |
0.06783 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | mutant enzyme N28A, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C | Severe acute respiratory syndrome-related coronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000 | Severe acute respiratory syndrome-related coronavirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | Severe acute respiratory syndrome-related coronavirus | - |
Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? | |
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | Severe acute respiratory syndrome-related coronavirus Tor2 | - |
Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Severe acute respiratory syndrome-related coronavirus Tor2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and Q-Sepharose column chromatography, and Superdex 75 gel filtration | Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | - |
Severe acute respiratory syndrome-related coronavirus | Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? | |
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | - |
Severe acute respiratory syndrome-related coronavirus Tor2 | Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34000 | Severe acute respiratory syndrome-related coronavirus |
Synonyms | Comment | Organism |
---|---|---|
coronavirus main protease | - |
Severe acute respiratory syndrome-related coronavirus |
SARS 3CLpro | - |
Severe acute respiratory syndrome-related coronavirus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | mutant enzyme N28A, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C | Severe acute respiratory syndrome-related coronavirus | |
0.643 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | wild type enzyme, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C | Severe acute respiratory syndrome-related coronavirus |