Cloned (Comment) | Organism |
---|---|
the catalytic domain of 3CLpro is synthesised chemically and cloned into a bacteriophage T7-based expression vector, pBT7. The plasmid DNA is transformed into Escherichia coli BL21 (DE3) for protein expression | Severe acute respiratory syndrome-related coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4',5,7,8-pentahydroxyflavone | i.e. herbacetin, efficiently blocks the enzymatic activity, suggested to be a template to design functionally improved inhibitors | Severe acute respiratory syndrome-related coronavirus | |
5,7-dihydroxy 4',6-dimethoxyflavone 7-rutinoside | i.e. pectolinarin, efficiently blocks the enzymatic activity, suggested to be a template to design functionally improved inhibitors | Severe acute respiratory syndrome-related coronavirus | |
apigenin-7-O-rhamnoglucoside | i.e. rhoifolin, efficiently blocks the enzymatic activity, suggested to be a template to design functionally improved inhibitors | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | P0C6X7 | human, i.e. SARS-CoV | - |
Purification (Comment) | Organism |
---|---|
- |
Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | - |
Severe acute respiratory syndrome-related coronavirus | Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SARS-CoV 3CL protease | - |
Severe acute respiratory syndrome-related coronavirus |
SARS-CoV nsp5-pp1a/pp1ab 3CL protease | - |
Severe acute respiratory syndrome-related coronavirus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.03317 | - |
pH 6.5, 37°C | Severe acute respiratory syndrome-related coronavirus | 3,4',5,7,8-pentahydroxyflavone | |
0.03745 | - |
pH 6.5, 37°C | Severe acute respiratory syndrome-related coronavirus | apigenin-7-O-rhamnoglucoside | |
0.03778 | - |
pH 6.5, 37°C | Severe acute respiratory syndrome-related coronavirus | 5,7-dihydroxy 4',6-dimethoxyflavone 7-rutinoside |