Literature summary for 3.4.22.8 extracted from

Cole, P.W.; Murakami, K.; Inagami, T.
Specificity and mechanism of clostripain catalysis (1971), Biochemistry, 10, 4246-4252.

Search for more literature for 3.4.22.8

Inhibitors

Inhibitors	Comment	Organism	Structure
benzamidine	-	Hathewaya histolytica
Butylamine	-	Hathewaya histolytica
butylguanidine	-	Hathewaya histolytica
ethylamine	-	Hathewaya histolytica
Ethylguanidine	-	Hathewaya histolytica
methylamine	-	Hathewaya histolytica
methylguanidine	-	Hathewaya histolytica
N-Alpha-p-tosyl-L-homoarginine methyl ester	-	Hathewaya histolytica
N-Alpha-p-tosyl-L-norarginine methyl ester	-	Hathewaya histolytica
Propylamine	-	Hathewaya histolytica
propylguanidine	-	Hathewaya histolytica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	effect of pH-value on Km-value for the hydrolysis of alpha-benzoyl-Arg ethyl ester, effect of temperature on Km-values	Hathewaya histolytica
0.022	-	p-toluenesulfonyl-L-Arg methyl ester	-	Hathewaya histolytica
0.24	0.25	alpha-benzoyl-Arg ethyl ester	depending on assay method	Hathewaya histolytica
0.25	-	Nalpha-p-tosyl-L-Arg amide	-	Hathewaya histolytica
0.26	-	p-Nitrophenyl p-guanidinobenzoate	-	Hathewaya histolytica
0.8	-	p-toluenesulfonyl-L-Arg methyl ester	-	Hathewaya histolytica
1.3	-	N-benzoyl-L-Arg amide	-	Hathewaya histolytica
3	-	Nalpha-benzoyl-L-Lys methyl ester	-	Hathewaya histolytica

Organism

Organism	UniProt	Comment	Textmining
Hathewaya histolytica	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-benzoyl-Arg amide + H2O	-	Hathewaya histolytica	N-benzoyl-Arg + NH3	-	?
N-benzoyl-Arg ethyl ester + H2O	-	Hathewaya histolytica	N-benzoyl-Arg + ethanol	-	?
Nalpha-benzoyl-Arg p-nitroanilide + H2O	-	Hathewaya histolytica	Nalpha-benzoyl-Arg + p-nitroaniline	-	?
Nalpha-benzoyl-L-Lys methyl ester + H2O	-	Hathewaya histolytica	Nalpha-benzoyl-L-Lys + methanol	-	?
Nalpha-benzoyl-Lys methyl ester + H2O	-	Hathewaya histolytica	Nalpha-benzoyl-Lys + methanol	-	?
Nalpha-p-tosyl-Arg amide + H2O	-	Hathewaya histolytica	Nalpha-p-tosyl-Arg + NH3	-	?
p-nitrophenyl p-guanidinobenzoate + H2O	-	Hathewaya histolytica	p-nitrophenol + p-guanidinobenzoate	-	?
p-tosyl-Arg methyl ester + H2O	-	Hathewaya histolytica	p-tosyl-Arg + methanol	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	effect of pH-value on the turnover-number for hydrolysis of alpha-benzoyl-Arg ethyl ester, effect of temperature on turnover numbers	Hathewaya histolytica
9.8	-	Nalpha-p-tosyl-L-Arg methyl ester	-	Hathewaya histolytica
10	-	Nalpha-p-tosyl-L-Arg amide	-	Hathewaya histolytica
22	-	Nalpha-benzoyl-L-Lys methyl ester	-	Hathewaya histolytica
31	-	p-Nitrophenyl p-guanidinobenzoate	-	Hathewaya histolytica
86	-	p-toluenesulfonyl-L-Arg methyl ester	-	Hathewaya histolytica
87	-	N-benzoyl-L-Arg amide	-	Hathewaya histolytica
91	92	alpha-benzoyl-Arg ethyl ester	depending on assay method	Hathewaya histolytica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	hydrolysis of alpha-benzoyl-Arg ethyl ester	Hathewaya histolytica

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Release 2023.1 (January 2023)