Literature summary for 3.4.22.B14 extracted from

Teng, H.; Pinon, J.D.; Weiss, S.R.
Expression of murine coronavirus recombinant papain-like proteinase: efficient cleavage is dependent on the lengths of both the substrate and the proteinase polypeptide (1999), J. Virol., 73, 2658-2666.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli by using a T7 RNA polymerase promoter system or as a maltose-binding protein fusion protein. With both overexpression systems the recombinant PLP-1 exhibits trans cleavage activity. When PLP-1 is expressed as a polypeptide that includes additional viral sequences at the carboxyl terminus of the predicted PLP-1 domain, a fivefold increase in proteolytic activity is observed	Murine coronavirus

Cloned (Comment)

Organism

expression in Escherichia coli by using a T7 RNA polymerase promoter system or as a maltose-binding protein fusion protein. With both overexpression systems the recombinant PLP-1 exhibits trans cleavage activity. When PLP-1 is expressed as a polypeptide that includes additional viral sequences at the carboxyl terminus of the predicted PLP-1 domain, a fivefold increase in proteolytic activity is observed

Murine coronavirus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ORF 1a polyprotein + H2O	Murine coronavirus	cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle	?	-	?
ORF 1a polyprotein + H2O	Murine coronavirus JHM	cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Murine coronavirus	P0C6V1	i.e. mouse hepatitis virus	-
Murine coronavirus JHM	P0C6V1	i.e. mouse hepatitis virus	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Murine coronavirus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da	Murine coronavirus	?	-	?
additional information	viral substrates translated from pSPDELTAMscN1S1, p28, p43 and p70, efficient cleavage requires a substrate greater than 69000 Da	Murine coronavirus JHM	?	-	?
ORF 1a polyprotein + H2O	cleavage in cis and in trans	Murine coronavirus	?	-	?
ORF 1a polyprotein + H2O	cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle	Murine coronavirus	?	-	?
ORF 1a polyprotein + H2O	cleavage in cis and in trans	Murine coronavirus JHM	?	-	?
ORF 1a polyprotein + H2O	cleavage in cis and in trans. PLP-1 cleavage activity appears to be optimal when both enzyme and substrate are present as part of polyproteins, as they are in the virus replication cycle	Murine coronavirus JHM	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	reaction is significantly more efficient at 22°C than when carried out at 30°C	Murine coronavirus