Cloned (Comment) | Organism |
---|---|
SENP1, recombinant expression of wild-type and mutant enzymes | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C599S | site-directed mutagenesis, the active site mutant precipitates SUMO1ylated proteins but not SUMO2/3-modified substrate | Mus musculus |
additional information | construction of several allelic defective enzyme mutants and a truncated mutant Senp1, overview, and a Senp1-beta-geo fusion protein, Expression of the amino half of Senp1 reduces Senp2 levels | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | inhibits desumoylation | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
SUMO1ylated protein + H2O | Mus musculus | main substrate | SUMO1 + protein | - |
? | |
SUMO2ylated protein + H2O | Mus musculus | - |
SUMO2 + protein | - |
? | |
SUMO3ylated protein + H2O | Mus musculus | - |
SUMO3 + protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
129S6 genetic background or mixed 129S6/C57Bl6 mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant enzyme Senp1 can deconjugate terminal Sumo1 moieties on poly-Sumo2/3 chains | Mus musculus | ? | - |
? | |
SUMO1ylated protein + H2O | - |
Mus musculus | SUMO1 + protein | - |
? | |
SUMO1ylated protein + H2O | main substrate | Mus musculus | SUMO1 + protein | - |
? | |
SUMO2ylated protein + H2O | - |
Mus musculus | SUMO2 + protein | - |
? | |
SUMO3ylated protein + H2O | - |
Mus musculus | SUMO3 + protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SENP1 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | embryos homozygous for this mutant allele, Senp1M1Mku show phenotypic abnormalities beginning at embryonic day (E) 11.5 with the majority dying between E12.5 and E14.5. Enzyme defects lead to accumulation of Sumo1-modified proteins in homozygous embryos, a lethal process, phenotypes, overview. Senp1Gt mutants express a truncated Senp1 affecting Sumo2/3 levels | Mus musculus |
physiological function | enzyme Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo, overview. Senp1 is the primary activity for desumoylation of Sumo1-modified proteins | Mus musculus |