Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | 5 mM, 49% inhibition | Saccharolobus solfataricus | |
additional information | no inhibition by 5 mM EDTA, 0.01 mM pepstatin A or 0.1 mM E-64 | Saccharolobus solfataricus | |
N-ethylmaleimide | 5 mM, 95% inhibition | Saccharolobus solfataricus | |
PMSF | 3 mM, 16% inhibition | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Saccharolobus solfataricus | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
gel filtration | Saccharolobus solfataricus |
11072 | - |
1 * 11072, calculated from sequence | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bacillus stearothermophilus alcohol dehydrogenase + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
Bacillus stearothermophilus alcohol dehydrogenase + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
L-Glu-7-amido-4-methylcoumarin + H2O | - |
Saccharolobus solfataricus | L-Glu + 7-amino-4-methylcoumarin | - |
? | |
L-Glu-7-amido-4-methylcoumarin + H2O | - |
Saccharolobus solfataricus P2 | L-Glu + 7-amino-4-methylcoumarin | - |
? | |
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O | 14% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus | N-acetyl-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin + H2O | - |
? | |
N-benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | 7.1% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus | N-benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O | 9.5% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus | N-benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus | N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus P2 | N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Ala-Glu-7-amido-4-methylcoumarin + H2O | 81% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus | N-succinyl-Ala-Glu + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O | 11% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus | N-succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O | 11% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus P2 | N-succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus | N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin | Saccharolobus solfataricus P2 | N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 10000, SDS-PAGE | Saccharolobus solfataricus |
monomer | 1 * 11072, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
peptidase C61.001 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | 60°C: about 75% of maximal activity, 80°C: about 50% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
90 min, prior heating of the protease solution stimulats the hydrolysis rate | Saccharolobus solfataricus |
80 | - |
30 min, prior heating of the protease solution stimulats the hydrolysis rate | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0: about 80% of maximal activity, pH 8.5: about 40% of maximal activity | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | calculated from sequence | - |
10.5 |