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Literature summary for 3.4.22.B77 extracted from

  • Guagliardi, A.; Cerchia, L.; Rossi, M.
    An intracellular protease of the crenarchaeon Sulfolobus solfataricus, which has sequence similarity to eukaryotic peptidases of the CD clan (2002), Biochem. J., 368, 357-363.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
DTNB 5 mM, 49% inhibition Saccharolobus solfataricus
additional information no inhibition by 5 mM EDTA, 0.01 mM pepstatin A or 0.1 mM E-64 Saccharolobus solfataricus
N-ethylmaleimide 5 mM, 95% inhibition Saccharolobus solfataricus
PMSF 3 mM, 16% inhibition Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Saccharolobus solfataricus 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10000
-
gel filtration Saccharolobus solfataricus
11072
-
1 * 11072, calculated from sequence Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bacillus stearothermophilus alcohol dehydrogenase + H2O
-
Saccharolobus solfataricus ?
-
?
Bacillus stearothermophilus alcohol dehydrogenase + H2O
-
Saccharolobus solfataricus P2 ?
-
?
L-Glu-7-amido-4-methylcoumarin + H2O
-
Saccharolobus solfataricus L-Glu + 7-amino-4-methylcoumarin
-
?
L-Glu-7-amido-4-methylcoumarin + H2O
-
Saccharolobus solfataricus P2 L-Glu + 7-amino-4-methylcoumarin
-
?
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O 14% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-acetyl-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin + H2O
-
?
N-benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O 7.1% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
?
N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O 9.5% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
?
N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus P2 N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
?
N-succinyl-Ala-Glu-7-amido-4-methylcoumarin + H2O 81% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Ala-Glu + 7-amino-4-methylcoumarin
-
?
N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O 11% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
?
N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O 11% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus P2 N-succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
?
N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus P2 N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 10000, SDS-PAGE Saccharolobus solfataricus
monomer 1 * 11072, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
peptidase C61.001
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 60°C: about 75% of maximal activity, 80°C: about 50% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
90 min, prior heating of the protease solution stimulats the hydrolysis rate Saccharolobus solfataricus
80
-
30 min, prior heating of the protease solution stimulats the hydrolysis rate Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0: about 80% of maximal activity, pH 8.5: about 40% of maximal activity Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus calculated from sequence
-
10.5