Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Shewanella amazonensis |
Protein Variants | Comment | Organism |
---|---|---|
D37A | the mutant is completely inactive towards the fluorogenic substrate Mca-KKPAEFFALK-Dnp at pH 4.0 | Shewanella amazonensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | 97.2 residual activity at 0.01 mM | Shewanella amazonensis | |
bestatin | 92.7 residual activity at 0.01 mM | Shewanella amazonensis | |
dithiothreitol | 91.2 residual activity at 2 mM | Shewanella amazonensis | |
E-64 | 89.5 residual activity at 0.01 mM | Shewanella amazonensis | |
EDTA | 82.8 residual activity at 5 mM | Shewanella amazonensis | |
iodoacetamide | 82.1 residual activity at 0.05 mM | Shewanella amazonensis | |
leupeptin | 91.9 residual activity at 0.01 mM | Shewanella amazonensis | |
pefabloc | 87.8 residual activity at 1 mM | Shewanella amazonensis | |
pepstatin A | complete inhibition at 0.001 mM | Shewanella amazonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
Mca-KKPAEFFALK-Dnp | at pH 4.0 and 37°C | Shewanella amazonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Shewanella amazonensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella amazonensis | A1S3N8 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Shewanella amazonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mca-KKPAEFFALK-Dnp + H2O | this peptide is preferentially cleaved at Phe-Phe, with a second minor cleavage occurring at Phe-Ala | Shewanella amazonensis | Mca-KKPAEF + FFALK-Dnp | - |
? | |
Mca-KLHPEVLFVLEK-Dnp + H2O | the preferred cleavage site is between Leu-Phe, a minor cleavage site is between Phe-Ala | Shewanella amazonensis | Mca-KLHPEVL + FVLEK-Dnp | - |
? | |
additional information | Edans-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys-Dabcyl-Arg, Arg-Glu-Edans-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys-Dabcyl-Arg, and Mca-Lys-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-Dnp are not significantly processed by shewasin A | Shewanella amazonensis | ? | - |
? | |
oxidized insulin B chain + H2O | two major cleavage sites are at Leu15-Tyr16 and Tyr16-Leu17 | Shewanella amazonensis | FVNQHLCGSHLVEAL + L-Tyr + LVCGERGFFYTPKA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Shewanella amazonensis |
Synonyms | Comment | Organism |
---|---|---|
shewasin A | - |
Shewanella amazonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | 50 | maximum activity is observed at temperatures between 42 and 50°C, decreasing so drastically above 50°C that complete loss of activity is detected at 60°C | Shewanella amazonensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Shewanella amazonensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Mca-KKPAEFFALK-Dnp | at pH 4.0 and 37°C | Shewanella amazonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | 4.5 | - |
Shewanella amazonensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5 | 50% activity is retained at pH 5.0 At pH 6.0, the enzyme shows no activity | Shewanella amazonensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
Mca-KKPAEFFALK-Dnp | at pH 4.0 and 37°C | Shewanella amazonensis |