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Literature summary for 3.4.23.25 extracted from
- Purification and properties of proteinase A from yeast (1980), J. Biol. Chem., 255, 12087-12093.
General Stability
| General Stability | Organism |
|---|---|
| Quite stable in concentrated solutions of urea, retains full activity after 1 h at 4°C in 6 M urea at pH 6.0, significant loss of activity at pH 5.0 and 7.0 | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Saccharomyces cerevisiae |  |
| Diazoacetyl-DL-norleucine methyl ester | in presence of copper ions | Saccharomyces cerevisiae | |
| pepstatin | - |
Saccharomyces cerevisiae | |
| Specific proteinase inhibitor from yeast | - |
Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41400 | - |
sedimentation-diffusion equilibrium ultracentrifugation | Saccharomyces cerevisiae |
| 41700 | - |
1 * 41700, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 7.5% w/w mannose and 1% of glucosamine and galactosamine | Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| casein + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| Hemoglobin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41700, Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 4-6: stable, pH 3: 80% loss of activity | Saccharomyces cerevisiae |
| 45 | - |
pH 5.5, 2 h, stable below | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
hemoglobin | Saccharomyces cerevisiae |
| 6 | - |
casein, azocasein | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
25°C, 48 h, 80% loss of activity | Saccharomyces cerevisiae |
| 4 | 6 | 25°C, stable | Saccharomyces cerevisiae |
| 5.5 | - |
2 h, stable below 45°C | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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