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Literature summary for 3.4.23.25 extracted from
- Purification and characterisation of proteases A and D from Debaryomyces hansenii (2008), Int. J. Food Microbiol., 124, 135-141.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | possibly involved in ripening processes of fermented meat products | Debaryomyces hansenii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Debaryomyces hansenii |  |
| DTT | - |
Debaryomyces hansenii | |
| HgCl2 | complete inhibition at 0.05 mM | Debaryomyces hansenii | |
| iodoacetate | weak inhibitor | Debaryomyces hansenii | |
| leupeptin | strong inhibitor | Debaryomyces hansenii | |
| p-chloromercuribenzoic acid | complete inhibition at 0.1 mM | Debaryomyces hansenii | |
| pepstatin A | - |
Debaryomyces hansenii | |
| PMSF | weak inhibitor | Debaryomyces hansenii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
1 * 42000, SDS-PAGE, native mass by gel filtration | Debaryomyces hansenii |
| 55000 | - |
gel filtration | Debaryomyces hansenii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Debaryomyces hansenii | - |
- |
- |
| Debaryomyces hansenii CECT12487 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Debaryomyces hansenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Debaryomyces hansenii | N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? | |
| N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Debaryomyces hansenii CECT 12487 | N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? | |
| N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Debaryomyces hansenii CECT12487 | N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE, native mass by gel filtration | Debaryomyces hansenii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PRA | - |
Debaryomyces hansenii |
| Protease A | aspartic protease | Debaryomyces hansenii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Debaryomyces hansenii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | very low activity at other pH | Debaryomyces hansenii |
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Release 2023.1 (January 2023)
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