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Literature summary for 3.4.23.49 extracted from

  • Mangel, W.F.; Toledo, D.L.; Brown, M.T.; Worzalla, K.; Lee, M.; Dunn, J.J.
    Omptin: an Escherichia coli outer membrane proteinase that activates plasminogen (1994), Methods Enzymol., 244, 384-399.
    View publication on PubMed
Search for more literature for 3.4.23.49

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
arginine
-
 Escherichia coli
benzamidine
-
 Escherichia coli
Cu2+
-
 Escherichia coli
diisopropylfluorophosphate
-
 Escherichia coli
Fe2+
-
 Escherichia coli
NaCl optimal activity in absence of NaCl, 80% inhibition by 0.15 M NaCl Escherichia coli
Zn2+ uncompetitive, pH-dependent; ZnCl2 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000058
-
 plasminogen pH 5.0 Escherichia coli
0.0036
-
 plasminogen pH 7.4 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
outer membrane
-
 Escherichia coli 19867
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plasminogen + H2O cleavage at an Arg-Val bond Escherichia coli heavy and light chain of plasmin + ?
-
 ?
Recombinant human gamma-interferon + H2O cleavage between basic amino acids Escherichia coli ?
-
 ?
T7 RNA polymerase + H2O cleavage at Lys-Arg173, Lys-Lys180, and Arg-Lys392 Escherichia coli ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 45 37°C: 40% of maximal activity, 45°C: maximal activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 plasminogen Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 6 4: 95% of maximal activity, 6.0: 25% of maximal activity Escherichia coli