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Literature summary for 3.4.23.49 extracted from

  • Zanfardino, A.; Pizzo, E.; Di Maro, A.; Varcamonti, M.; DAlessio, G.
    The bactericidal action on Escherichia coli of ZF-RNase-3 is triggered by the suicidal action of the bacterium OmpT protease (2010), FEBS J., 277, 1921-1928.
    View publication on PubMed
Search for more literature for 3.4.23.49

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 significant inhibition at 1 mM Escherichia coli DH5[alpha]
ZnCl2 significant inhibition at 1 mM Escherichia coli DH5[alpha]

Organism

Organism UniProt Comment Textmining
Escherichia coli DH5-alpha
-
-
-
Escherichia coli DH5[alpha]
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ZF-RNase-3 + H2O when ZF-RNase-3 is added to Escherichia coli cultures, it is cleaved at a specific Arg-Arg peptide bond, thus engendering two peptide fragments. The larger fragment (residues 31-124), produced by proteolysis and reduction of a disulfide, is recognized as the actual bactericidal agent Escherichia coli DH5[alpha] ?
-
 ?
ZF-RNase-3 + H2O when ZF-RNase-3 is added to Escherichia coli cultures, it is cleaved at a specific Arg-Arg peptide bond, thus engendering two peptide fragments. The larger fragment (residues 31-124), produced by proteolysis and reduction of a disulfide, is recognized as the actual bactericidal agent Escherichia coli DH5-alpha ?
-
 ?

Synonyms

Synonyms Comment Organism
ompT
-
 Escherichia coli DH5[alpha]
omptin protease
-
 Escherichia coli DH5[alpha]

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli DH5[alpha]

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli DH5[alpha]

General Information

General Information Comment Organism
malfunction Escherichia coli BL21(DE3) strain, which does not possess the ompT gene, no proteolysis of ZF-RNase-3 is observed Escherichia coli DH5[alpha]