Literature summary for 3.4.24.19 extracted from

Hojima, Y.; van der Rest, M.; Prockop, P.J.
Type I procollagen carboxyl-terminal proteinase from chick embryo tendons. Purification and characterization (1985), J. Biol. Chem., 260, 15996-16003.

General Stability

General Stability	Organism
Ca2+ stabilizes against thermal inactivation	Gallus gallus
Lyophilization overnight, 50% loss of activity	Gallus gallus

Inhibitors	Comment	Organism
(NH4)2SO4	-	Gallus gallus
1,10-phenanthroline	-	Gallus gallus
1,4-dithio-L-threitol	-	Gallus gallus
2,2'-dipyridyl	-	Gallus gallus
2-mercaptoethanol	-	Gallus gallus
Bovine alpha2-macroglobulin	-	Gallus gallus
Cd2+	CdCl2	Gallus gallus
Chicken alpha2-macroglobulin	-	Gallus gallus
chymostatin	-	Gallus gallus
CoCl2	-	Gallus gallus
concanavalin A	-	Gallus gallus
CuCl2	-	Gallus gallus
diisopropylphosphofluoridate	weak	Gallus gallus
EDTA	-	Gallus gallus
EGTA	-	Gallus gallus
Fetal bovine serum	weak	Gallus gallus
glycine	weak	Gallus gallus
L-alanine	weak	Gallus gallus
L-arginine	weak	Gallus gallus
L-lysine	-	Gallus gallus
metal chelators	-	Gallus gallus
MnCl2	-	Gallus gallus
additional information	not: cysteine proteinases; not: inhibitors of serine	Gallus gallus
Na2SO4	-	Gallus gallus
Na3PO4	-	Gallus gallus
NH4Cl	weak	Gallus gallus
NiCl2	-	Gallus gallus
Ovostatin	weak	Gallus gallus
pepstatin	A	Gallus gallus
phosphoramidon	weak	Gallus gallus
PMSF	weak	Gallus gallus
reduced glutathione	-	Gallus gallus
SDS	-	Gallus gallus
ZnCl2	-	Gallus gallus

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000096	-	Procollagen type I	-	Gallus gallus

Metals/Ions	Comment	Organism	Structure
Ca2+	dependent on, 5-10 mM required for optimal activity	Gallus gallus

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
97000	110000	gel filtration, PAGE	Gallus gallus
102000	-	1 * 102000, chicken, SDS-PAGE	Gallus gallus

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Procollagen type I + H2O	Gallus gallus	enzyme is involved in the processing of type I procollagen	?	-	?

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-

Purification (Comment)	Organism
-	Gallus gallus

Source Tissue	Comment	Organism	Textmining
culture medium	of 17-day-old embryo tendons	Gallus gallus	-

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Gallus gallus

Storage Stability	Organism
4°C, -20°C or -70°C in 0.3 M Tris-HCl, 1.5 M NaCl, 5 mM CaCl2, 0.02% Brij 35, 0.01% NaN3, pH 7.5, less than 25% loss of activity after 2 months	Gallus gallus

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Procollagen type I + H2O	specifically cleaves an Ala-Asp bond in both the pro-alpha1(I) and pro-alpha2(I) chains	Gallus gallus	Type I pNcollagen + C-propeptides	-	?
Procollagen type I + H2O	specifically cleaves carboxyl-terminal propeptides of a homotrimer of pro-alpha1(I) chains and type II and III procollagens, but it does not cleave type IV procollagen	Gallus gallus	Type I pNcollagen + C-propeptides	-	?
Procollagen type I + H2O	enzyme is involved in the processing of type I procollagen	Gallus gallus	?	-	?

Subunits	Comment	Organism
monomer	1 * 102000, chicken, SDS-PAGE	Gallus gallus

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	assay at	Gallus gallus

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	8.5	-	Gallus gallus

pH Minimum	pH Maximum	Comment	Organism
7.5	8.5	7.5: about 80% of activity maximum, 8-8.5: activity maximum	Gallus gallus

pH Stability	pH Stability Maximum	Comment	Organism
4.3	-	1 h, in presence of 5 mM CaCl2, more than 80% loss of activity	Gallus gallus
4.8	-	1 h, in presence of 5 mM CaCl2, more than 80% loss of activity	Gallus gallus
6	9	1 h, in presence of 5 mM CaCl2, less than 20% loss of activity	Gallus gallus