BRENDA - Enzyme Database show
show all sequences of 3.4.24.28

A neutral protease from Bacillus nematocida, another potential virulence factor in the infection against nematodes

Niu, Q.; Huang, X.; Zhang, L.; Li, Y.; Li, J.; Yang, J.; Zhang, K.; Arch. Microbiol. 185, 439-448 (2006)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Al3+
-
Bacillus nematocida
dithiothreitol
5 mM, 83% residual activity
Bacillus nematocida
Hg2+
-
Bacillus nematocida
phenylmethylsulfonyl fluoride
0.1 mM, 77% residual activity
Bacillus nematocida
Zn2+
-
Bacillus nematocida
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bacillus nematocida
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
100 mM, 60% activation
Bacillus nematocida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Bacillus nematocida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus nematocida
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
55.6
-
pH 6.5, 55C
Bacillus nematocida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Bovine serum albumin + H2O
8.7% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Collagen + H2O
1.3% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Denatured casein + H2O
-
667347
Bacillus nematocida
?
-
-
-
?
denatured collagen + H2O
11.5% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Gelatin + H2O
9% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
nematode cuticle + H2O
2.8% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
skimmed milk + H2O
60% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Bacillus nematocida
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Bacillus nematocida
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
60
stable for 30 min
Bacillus nematocida
70
-
1 h, complete inactivation
Bacillus nematocida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Bacillus nematocida
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
11
decrease of activity from pH 6.5 to 11
Bacillus nematocida
11
-
no residual activity
Bacillus nematocida
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
1h, less than 40% remaining activity
Bacillus nematocida
5
8
stable within this range
Bacillus nematocida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Al3+
-
Bacillus nematocida
dithiothreitol
5 mM, 83% residual activity
Bacillus nematocida
Hg2+
-
Bacillus nematocida
phenylmethylsulfonyl fluoride
0.1 mM, 77% residual activity
Bacillus nematocida
Zn2+
-
Bacillus nematocida
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Bacillus nematocida
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
100 mM, 60% activation
Bacillus nematocida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Bacillus nematocida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
55.6
-
pH 6.5, 55C
Bacillus nematocida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Bovine serum albumin + H2O
8.7% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Collagen + H2O
1.3% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Denatured casein + H2O
-
667347
Bacillus nematocida
?
-
-
-
?
denatured collagen + H2O
11.5% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Gelatin + H2O
9% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
nematode cuticle + H2O
2.8% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
skimmed milk + H2O
60% of the activity with denatured casein
667347
Bacillus nematocida
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Bacillus nematocida
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
-
Bacillus nematocida
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
60
stable for 30 min
Bacillus nematocida
70
-
1 h, complete inactivation
Bacillus nematocida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Bacillus nematocida
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
11
decrease of activity from pH 6.5 to 11
Bacillus nematocida
11
-
no residual activity
Bacillus nematocida
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
1h, less than 40% remaining activity
Bacillus nematocida
5
8
stable within this range
Bacillus nematocida
Other publictions for EC 3.4.24.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733469
Saxena
MALDI-TOF MS and CD spectral a ...
Bacillus cereus, Bacillus cereus B80
BioMed Res. Int.
2015
527015
2015
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1
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-
-
-
1
-
1
1
-
1
2
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1
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1
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11
1
1
1
4
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1
1
2
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1
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1
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1
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1
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11
1
1
1
4
-
1
1
2
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1
1
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-
733978
Guinane
-
Generation of the antimicrobia ...
Bacillus subtilis
Int. Dairy J.
49
1-7
2015
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1
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734363
Hatta
-
Bacillolysin, papain, and subt ...
Bacillus amyloliquefaciens
J. Cereal Sci.
61
41-47
2015
-
1
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733130
Wang
Purification and characterizat ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens SYB-001
Appl. Biochem. Biotechnol.
170
2021-2033
2013
2
1
-
-
-
-
11
1
-
5
2
2
-
4
-
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1
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-
-
1
-
2
1
1
1
2
-
1
1
4
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2
1
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-
-
-
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-
11
-
1
-
5
2
2
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1
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-
1
-
2
1
1
1
2
-
1
1
4
-
-
-
-
-
-
-
733167
Luo
Bacillus thuringiensis metallo ...
Bacillus thuringiensis, Bacillus thuringiensis YBT-1518
Appl. Environ. Microbiol.
79
460-468
2013
-
-
1
-
-
-
8
-
-
3
-
2
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5
-
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1
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4
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1
1
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1
1
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1
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8
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3
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1
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4
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1
1
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1
1
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2
2
-
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-
733545
Zhu
Optimization of neutral protea ...
Bacillus subtilis, Bacillus subtilis DES-59
Biotechnol. Appl. Biochem.
60
336-342
2013
-
-
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7
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4
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4
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718012
Niu
Overexpression of the key viru ...
Hathewaya histolytica, Paenibacillus polymyxa
J. Mol. Microbiol. Biotechnol.
21
130-137
2011
-
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2
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2
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9
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2
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6
2
2
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2
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6
2
2
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711482
Duerrschmidt
Refolding of the non-specific ...
Geobacillus stearothermophilus
Biophys. Chem.
147
66-73
2010
-
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1
1
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2
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2
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2
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717233
Rudakova
Characteristics of a novel sec ...
Bacillus intermedius
Biochemistry (Moscow)
75
1294-1301
2010
-
-
1
-
-
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10
1
1
3
1
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3
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1
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7
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8
1
1
1
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1
1
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1
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1
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10
-
1
1
3
1
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1
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7
-
8
1
1
1
-
1
1
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1
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1
1
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701210
Zhang
Expression, purification, and ...
Geobacillus stearothermophilus
Sci. China C Life Sci.
51
52-59
2008
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1
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1
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1
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1
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1
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1
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1
1
1
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1
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1
2
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685711
Mansfeld
The stability of engineered th ...
Geobacillus stearothermophilus
Biotechnol. Bioeng.
97
672-679
2007
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1
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1
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1
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8
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2
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3
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687106
Albillos
Analysis by capillary electrop ...
Bacillus subtilis
Int. Dairy J.
17
1195-1200
2007
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667347
Niu
A neutral protease from Bacill ...
Bacillus nematocida
Arch. Microbiol.
185
439-448
2006
-
-
-
-
-
-
5
-
1
1
1
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-
8
-
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1
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7
1
1
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2
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1
2
2
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5
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1
1
1
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1
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7
1
1
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2
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1
2
2
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668546
Duerrschmidt
An engineered disulfide bridge ...
Geobacillus stearothermophilus
FEBS J.
272
1523-1534
2005
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2
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669317
Narasaki
Bacillolysin MA, a novel bacte ...
Bacillus megaterium, Bacillus megaterium A9542
J. Biol. Chem.
280
14278-14287
2005
1
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3
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670771
Mansfeld
The propeptide is not required ...
Geobacillus stearothermophilus
Protein Expr. Purif.
39
219-228
2005
-
1
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651101
Duerrschmidt
Differentiation between confor ...
Geobacillus stearothermophilus
Eur. J. Biochem.
268
3612-3618
2001
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1
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1
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1
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1
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1
1
1
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650655
Van den Burg
-
Rendering one autolysis site i ...
Bacillus subtilis
Biotechnol. Appl. Biochem.
27
125-132
1998
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1
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1
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4
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1
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1
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4
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1
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1
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4
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1
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4
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2
4
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31162
Tsuru
Zinc protease of Bacillus subt ...
Bacillus subtilis
J. Biochem.
113
101-105
1993
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1
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31151
Holland
Structural comparison suggests ...
Bacillus cereus
Biochemistry
31
11310-11316
1992
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31166
Paberit
-
Inhibition of thermolysin and ...
Brevibacillus brevis
Quant. Struct. -Act. Relat.
11
28-33
1992
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31157
Stoeva
Primary structure of a zinc pr ...
Bacillus pumilus, Bacillus pumilus 76
Biochemistry
29
527-534
1990
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31158
Pauptit
Crystal structure of neutral p ...
Bacillus cereus
J. Mol. Biol.
199
525-537
1988
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31154
Sidler
The primary structure of Bacil ...
Bacillus cereus, Bacillus subtilis
Biol. Chem. Hoppe-Seyler
367
643-657
1986
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31156
Takagi
Nucleotide sequence and promot ...
Geobacillus stearothermophilus
J. Bacteriol.
163
824-831
1985
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5
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31155
Vasantha
Genes for alkaline protease an ...
Bacillus amyloliquefaciens
J. Bacteriol.
159
811-819
1984
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4
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31161
Yang
Cloning of the neutral proteas ...
Bacillus subtilis
J. Bacteriol.
160
15-21
1984
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31163
Sidler
-
Isolation procedures for therm ...
Geobacillus stearothermophilus
Eur. J. Appl. Microbiol. Biotechnol.
10
197-209
1980
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31112
Holmquist
Esterase activity of zinc neut ...
Bacillus subtilis
Biochemistry
15
101-107
1976
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3
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31165
Sidler
Neutral proteases with differe ...
Geobacillus stearothermophilus
FEBS Lett.
25
292-294
1972
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31159
Feder
Bacillus cereus neutral protea ...
Bacillus cereus
Biochim. Biophys. Acta
251
74-78
1971
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2
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8
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31160
Millet
Specificity of megateriopeptid ...
Bacillus megaterium
Eur. J. Biochem.
9
456-462
1969
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31150
Morihara
Comparison of the specificitie ...
Bacillus subtilis
Arch. Biochem. Biophys.
123
572-588
1968
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