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Literature summary for 3.4.24.28 extracted from
- Overexpression of the key virulence proteases Bace16 and Bae16 in Bacillus nematocida B16 to improve its nematocidal activity (2011), J. Mol. Microbiol. Biotechnol., 21, 130-137.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Paenibacillus polymyxa | - |
- |
| extracellular | the enzyme is secreted | Hathewaya histolytica | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Paenibacillus polymyxa |
| 34000 | - |
x * 34000, SDS-PAGE | Hathewaya histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | - |
- |
- |
| Paenibacillus polymyxa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extracellular enzyme from culture medium by anion exchange chromatography | Paenibacillus polymyxa |
| extracellular enzyme from culture medium by several chromatographic steps including one affinity based step | Hathewaya histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Paenibacillus polymyxa | ? | - |
? |  |
| azocasein + H2O | - |
Hathewaya histolytica | ? | - |
? | |
| fluorescein isothyocyanate-conjugated bovine serum albumin + H2O | - |
Paenibacillus polymyxa | ? | - |
? | |
| fluorescein isothyocyanate-conjugated bovine serum albumin + H2O | - |
Hathewaya histolytica | ? | - |
? | |
| additional information | C histolyticum neutral protease, CHNP, shows similar preferences for specific amino acid sequences as thermolysin, EC 3.4.24.27, and Bacillus polymyxa protease, which cleave on the amino terminal side of hydrophobic amino acids such as leucine | Paenibacillus polymyxa | ? | - |
? | |
| additional information | C histolyticum neutral protease, CHNP, shows similar preferences for specific amino acid sequences as thermolysin, EC 3.4.24.27, and Bacillus polymyxa protease, which cleave on the amino terminal side of hydrophobic amino acids such as leucine | Hathewaya histolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Paenibacillus polymyxa |
| ? | x * 34000, SDS-PAGE | Hathewaya histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bacillus polymyxa protease | - |
Paenibacillus polymyxa |
| C histolyticum neutral protease | - |
Hathewaya histolytica |
| CHNP | - |
Hathewaya histolytica |
| Neutral protease | - |
Paenibacillus polymyxa |
| Neutral protease | - |
Hathewaya histolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Paenibacillus polymyxa |
| 35 | - |
assay at | Hathewaya histolytica |
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Release 2023.1 (January 2023)
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