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Literature summary for 3.4.24.28 extracted from

  • Wang, J.; Xu, A.; Wan, Y.; Li, Q.
    Purification and characterization of a new metallo-neutral protease for beer brewing from Bacillus amyloliquefaciens SYB-001 (2013), Appl. Biochem. Biotechnol., 170, 2021-2033.
    View publication on PubMed
Search for more literature for 3.4.24.28

Activating Compound

Activating Compound Comment Organism Structure
Tween-20 1.61fold activation at 50 mM Bacillus amyloliquefaciens
Tween-80 1.34fold activation at 50 mM Bacillus amyloliquefaciens

Application

Application Comment Organism
food industry in the beer brewing process, the neutral protease during mashing process can release more amino acids from wort such as aspartic acid, arginine, methione, and histidine, resulting in a better amino acid profile in wort Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 27% inhibition at 50 mM Bacillus amyloliquefaciens
Ba2+ 88% inhibition at 10 mM Bacillus amyloliquefaciens
Cu2+ 30% inhibition at 10 mM Bacillus amyloliquefaciens
dithio-bis-nitrobenzoic acid 64% inhibition at 50 mM Bacillus amyloliquefaciens
dithiothreitol 70% inhibition at 50 mM Bacillus amyloliquefaciens
EDTA complete inhibition at 50 mM, can be reversed by Zn2+ Bacillus amyloliquefaciens
Fe2+ 86% inhibition at 10 mM Bacillus amyloliquefaciens
Fe3+ 98% inhibition at 10 mM Bacillus amyloliquefaciens
SDS 37% inhibition at 50 mM Bacillus amyloliquefaciens
Triton X-100 slight inhibition at 50 mM Bacillus amyloliquefaciens
Zn2+ 19% inhibition at 10 mM Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information purified enzyme, Km value of 3.97 mg/ml and Vmax value of 657 U/mg/min of substrate casein Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 20% at 10 mM Bacillus amyloliquefaciens
Li+ activates 20% at 10 mM Bacillus amyloliquefaciens
Mg2+ activates 20% at 10 mM Bacillus amyloliquefaciens
Mn2+ activates 2.90fold at 10 mM Bacillus amyloliquefaciens
Zn2+ a zinc metalloprotease Bacillus amyloliquefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36800
-
 x * 37000, SDS-PAGE, x * 36800, about, mass spectrometry Bacillus amyloliquefaciens
37000
-
 x * 37000, SDS-PAGE, x * 36800, about, mass spectrometry Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
casein + H2O Bacillus amyloliquefaciens
-
 ?
-
 ?
casein + H2O Bacillus amyloliquefaciens SYB-001
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
 isolated from soil
-
Bacillus amyloliquefaciens SYB-001
-
 isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 5.6fold from culture supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11280
-
 purified native enzyme, pH 7.0, 40°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
casein + H2O
-
 Bacillus amyloliquefaciens SYB-001 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE, x * 36800, about, mass spectrometry Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
metallo-neutral protease
-
 Bacillus amyloliquefaciens
Neutral protease
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range, 41% of maximal activity at 30°C, 50% at 70°C Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 purified extracellular enzyme, pH 7.0, about 70% activity remaining after 60 min, rapid loss of activity above 60°C Bacillus amyloliquefaciens
60
-
 purified extracellular enzyme, pH 7.0, loss of over 80% activity after 10 min, inactivation after 60 min Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Bacillus amyloliquefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 purified extracellular enzyme, 50°C, loss of 70% activity after 60 min Bacillus amyloliquefaciens
6
-
 purified extracellular enzyme, 50°C, no loss of activity after 60 min, stable at Bacillus amyloliquefaciens
7
-
 purified extracellular enzyme, 50°C, loss of 20% activity after 60 min Bacillus amyloliquefaciens
8 10 purified extracellular enzyme, 50°C, loss of 40% activity after 60 min Bacillus amyloliquefaciens