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Literature summary for 3.4.24.28 extracted from
- MALDI-TOF MS and CD spectral analysis for identification and structure prediction of a purified, novel, organic solvent stable, fibrinolytic metalloprotease from Bacillus cereus B80 (2015), BioMed Res. Int., 2015, 527015.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis | Bacillus cereus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus cereus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metalloprotease | Bacillus cereus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, SDS-PAGE | Bacillus cereus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | the enzyme exhibits strong fibrinolytic, collagenolytic, and gelatinolytic properties and stability in various organic solvents, overview | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus B80 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 13.1fold by ethanol precipitation, gel filtration, and anion exchange chromatography | Bacillus cereus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3556 | - |
purified enzyme, pH 7.5, 37°C | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Bovine serum albumin + H2O | - |
Bacillus cereus | ? | - |
? |  |
| Bovine serum albumin + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| casein + H2O | - |
Bacillus cereus | ? | - |
? | |
| casein + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| Collagen + H2O | - |
Bacillus cereus | ? | - |
? | |
| Collagen + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| fibrinogen + H2O | - |
Bacillus cereus | fibrin + ? | - |
? | |
| Gelatin + H2O | - |
Bacillus cereus | ? | - |
? | |
| Gelatin + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| additional information | the enzyme is able to hydrolyze various proteins with highest affinity towards casein followed by bovine serum albumin and gelatin | Bacillus cereus | ? | - |
? | |
| additional information | the enzyme is able to hydrolyze various proteins with highest affinity towards casein followed by bovine serum albumin and gelatin | Bacillus cereus B80 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, SDS-PAGE | Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | activity range | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified enzyme, 2 h, stable | Bacillus cereus |
| 40 | 50 | purified enzyme, 2 h, loss of 16-22% activity | Bacillus cereus |
| 60 | - |
purified enzyme, loss of 30% activity after 1 h, and loss of 56% activity after 2 h | Bacillus cereus |
| 70 | - |
purified enzyme, loss of 50% activity after 1 h | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | activity range | Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | purified enzyme, 60°C, loss of 20-25% activity after 10 min | Bacillus cereus |
| 10 | - |
purified enzyme, 60°C, loss of 40% activity after 10 min | Bacillus cereus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme exhibits 70-93% similarity with zinc metalloproteases from various strains Bacillus sp. specifically from Bacillus cereus group, 22-24% identity with bacillolysin from Bacillus cereus, mass spectrometry | Bacillus cereus |
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