General Stability | Organism |
---|---|
Rapid autolysis, Zn2+ as protease inhibitor protects, Ca2+ does not stabilize | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Enterococcus faecalis | |
diethylpyrocarbonate | partially reversed by hydroxylamine (not) | Enterococcus faecalis | |
EDTA | reversible by Co2+, Mn2+, Zn2+, Ca2+, Cu2+ or Mg2+ with decreasing order of efficiency, not by Cd2+, Ba2+, Ni2+ or Hg2+ | Enterococcus faecalis | |
L-cysteine | - |
Enterococcus faecalis | |
additional information | no inhibition by DTNB, carbodiimide/glycine ethyl ester; no inhibition by iodoacetate; no inhibition by PMSF, phenylglyoxal; no inhibition by tosyl-Leu chloromethyl ketone, tosyl-Phe chloromethyl ketone, trasylol (i.e. bovine lung trypsin inhibitor) | Enterococcus faecalis | |
NaBr | above 5 M, activates at 1-5 M | Enterococcus faecalis | |
p-chloromercuribenzoate | not | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
N-3-(2-furyl)acryloylglycyl-L-leucinamide | - |
Enterococcus faecalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Enterococcus faecalis | - |
- |
soluble | - |
Enterococcus faecalis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaBr | activation, 1-5 M, inhibits above | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, Streptococcus faecalis, SDS-PAGE | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Enterococcus faecalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Enterococcus faecalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Enterococcus faecalis |
Storage Stability | Organism |
---|---|
At very low temperatures stored in the presence of Zn2+ as protease inhibitor to prevent autolysis of purified enzyme | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine fibrinogen + H2O | B-chain and to a lesser extent A-chain | Enterococcus faecalis | ? | - |
? | |
additional information | No substrates are N-3-(2-furyl)acryloyl-Gly-Gly-Gly, N-3-(2-furyl)acryloyl-Gly-L-Leu or N-3-(2-furyl)acryloyl-Gly-L-Ala | Enterococcus faecalis | ? | - |
? | |
additional information | neutral metalloendopeptidase, no aminopeptidase or esterase activity | Enterococcus faecalis | ? | - |
? | |
N-3-(2-Furyl)acryloyl tripeptides derivatives + H2O | chromogenic substrates, e.g. -L-Ala-L-Ala-L-Ala, -Gly-L-Phe-L-Phe, -L-Phe-L-Phe-L-Phe, -Gly-L-Leu-L-Tyr, -Gly-L-Met-L-Leu, -L-Leu-L-Leu-L-Leu | Enterococcus faecalis | ? | - |
? | |
N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O | - |
Enterococcus faecalis | ? | - |
? | |
N-3-(2-Furyl)acryloyl-Gly-L-Phe + H2O | chromogenic substrate | Enterococcus faecalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, Streptococcus faecalis, SDS-PAGE | Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
spectrophotometric assay at | Enterococcus faecalis |
37 | - |
assay at | Enterococcus faecalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ca2+ reduces thermal stability | Enterococcus faecalis |
55 | - |
rapid inactivation above | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5 | Enterococcus faecalis |
6.5 | - |
N-3-(2-furyl)acryloyl-Gly-L-Leu amide as substrate | Enterococcus faecalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | about half-maximal activity at pH 5 and 7.5, N-3-(2-furyl)acryloyl-Gly-L-Leu amide as substrate | Enterococcus faecalis |