Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | low salt concentrations activate the proteolytic activity | Homo sapiens | |
Urea | activation, substrate is degraded at 1 M | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | weak | Homo sapiens | |
EDTA | complete inhibition | Homo sapiens | |
EGTA | complete inhibition | Homo sapiens | |
additional information | no inhibition by iodoacetamide, leupeptin, and serine protease inhibitorsDFP, PMSF, aprotinin | Homo sapiens | |
N-ethylmaleimide | slow and weak inhibition | Homo sapiens | |
Z-Phe-Phe-CHN2 | best peptidyl diazomethyl ketone inhibitor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | plasma | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | best activating metal ion | Homo sapiens | |
Ca2+ | activating | Homo sapiens | |
Mg2+ | slightly activating | Homo sapiens | |
additional information | no activtion by Zn2+, Cu2+, Cd2+, Ni2+, Co2+, Mn2+ | Homo sapiens | |
Sr2+ | activating | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
von Willebrand factor + H2O | Homo sapiens | cleavage of peptide bond Tyr842-Met843, large hemostatically active multimers are cleaved to smaller less active forms, increased proteolytic degradation in patients suffering from von Willebrand disease typ 2A | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
about 10000fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | not in platelet | Homo sapiens | - |
plasma | - |
Homo sapiens | - |
serum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with human fibrinogen, bovine serum albumin, and calf skin collagen | Homo sapiens | ? | - |
? | |
proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
von Willebrand factor + H2O | cleavage of peptide bond Tyr842-Met843 | Homo sapiens | 2 peptides | - |
? | |
von Willebrand factor + H2O | cleavage of peptide bond Tyr842-Met843, large hemostatically active multimers are cleaved to smaller less active forms, increased proteolytic degradation in patients suffering from von Willebrand disease typ 2A | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 x 300000, nonreducing SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
M12.241 | Merops-ID | Homo sapiens |
von-Willebrand factor degrading protease | - |
Homo sapiens |
vWF-degrading protease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
stable for several days during purification at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at neutral pH at low salt concentration | Homo sapiens |
8 | - |
in presence of Ba2+ | Homo sapiens |
9 | 10 | in presence of Ca2+ | Homo sapiens |