Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged HslU and HslUV in strain BB101 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Escherichia coli | |
0.004 | - |
Arc1-53-st11-titin-ssrA | pH 7.6, 37°C, HslUV | Escherichia coli | |
0.0052 | - |
Arc-MYL-st11 | pH 7.6, 37°C, HslUV | Escherichia coli | |
0.04 | - |
Arc-MYL-st11 plus | pH 7.6, 37°C, HslUV | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
additional information | HslU requires Mg2+ together with ATP for activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged HslU and HslUV from strain BB101 by nickel affinity and ion exchange chromatography, and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arc + H2O | repressor protein, specific degradation, especially at heat shock temperatures, recognition of sequences near the N-terminus of Arc and strong binding requiring Mg2+ and ATP for degradation | Escherichia coli | ? | - |
? | |
Arc-MYL-st11 + H2O | recombinant Arc fusion protein | Escherichia coli | ? | - |
? | |
Arc-MYL-st11 plus + H2O | recombinant Arc fusion protein | Escherichia coli | ? | - |
? | |
Arc1-53-st11-titin-ssrA + H2O | recombinant truncated Arc fusion protein | Escherichia coli | ? | - |
? | |
gt1 + H2O | substrate of HslU | Escherichia coli | ? | - |
? | |
additional information | substrate binding, ATP-dependent protein degradation, and reaction mechanism, substrate engagement must occur after ATP-binding before HslUV unfolds the proteins, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type enzyme | Escherichia coli |
monomer | recombinant mutant I37A, predominantly | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AAA+ HslUV protease | - |
Escherichia coli |
ClpYQ | - |
Escherichia coli |
HslUV | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
Arc-MYL-st11 plus | pH 7.6, 37°C, HslUV | Escherichia coli | |
0.11 | - |
Arc1-53-st11-titin-ssrA | pH 7.6, 37°C, HslUV | Escherichia coli | |
0.11 | - |
Arc-MYL-st11 | pH 7.6, 37°C, HslUV | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | required, ATP | Escherichia coli | |
additional information | HslU requires Mg2+ together with ATP for activity | Escherichia coli |