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Literature summary for 3.4.25.2 extracted from

  • Burton, R.E.; Baker, T.A.; Sauer, R.T.
    Nucleotide-dependent substrate recognition by the AAA+ HslUV protease (2005), Nat. Struct. Mol. Biol., 12, 245-251.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged HslU and HslUV in strain BB101 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Escherichia coli
0.004
-
Arc1-53-st11-titin-ssrA pH 7.6, 37°C, HslUV Escherichia coli
0.0052
-
Arc-MYL-st11 pH 7.6, 37°C, HslUV Escherichia coli
0.04
-
Arc-MYL-st11 plus pH 7.6, 37°C, HslUV Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
additional information HslU requires Mg2+ together with ATP for activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged HslU and HslUV from strain BB101 by nickel affinity and ion exchange chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arc + H2O repressor protein, specific degradation, especially at heat shock temperatures, recognition of sequences near the N-terminus of Arc and strong binding requiring Mg2+ and ATP for degradation Escherichia coli ?
-
?
Arc-MYL-st11 + H2O recombinant Arc fusion protein Escherichia coli ?
-
?
Arc-MYL-st11 plus + H2O recombinant Arc fusion protein Escherichia coli ?
-
?
Arc1-53-st11-titin-ssrA + H2O recombinant truncated Arc fusion protein Escherichia coli ?
-
?
gt1 + H2O substrate of HslU Escherichia coli ?
-
?
additional information substrate binding, ATP-dependent protein degradation, and reaction mechanism, substrate engagement must occur after ATP-binding before HslUV unfolds the proteins, overview Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer wild-type enzyme Escherichia coli
monomer recombinant mutant I37A, predominantly Escherichia coli

Synonyms

Synonyms Comment Organism
AAA+ HslUV protease
-
Escherichia coli
ClpYQ
-
Escherichia coli
HslUV
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.077
-
Arc-MYL-st11 plus pH 7.6, 37°C, HslUV Escherichia coli
0.11
-
Arc1-53-st11-titin-ssrA pH 7.6, 37°C, HslUV Escherichia coli
0.11
-
Arc-MYL-st11 pH 7.6, 37°C, HslUV Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP required, ATP Escherichia coli
additional information HslU requires Mg2+ together with ATP for activity Escherichia coli