Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoyl-Arg-His-D-Asp-CH2Cl | 1 mM, about 70% inhibition of paenidase I and II | Paenibacillus sp. | |
Co2+ | 1.0 mM, 31% inhibition of paenidase I, 46% inhibition of paenidase II | Paenibacillus sp. | |
Mn2+ | 1.0 mM MnCl2, 31% inhibition of paenidase I, 60% inhibition of paenidase II | Paenibacillus sp. | |
additional information | no inhibition: iodoacetic acid, p-chloromercuribenzoate, EDTA, 1,10-phenanthroline, amastatin, antipain, diisopropylfluorophosphate, soybean trypsin inhibitor | Paenibacillus sp. | |
NEM | 0.1 mM, about 10% inhibition | Paenibacillus sp. | |
pepstatin | 0.01 mg/ml, about 50% inhibition of paenidase I and II | Paenibacillus sp. | |
Zn2+ | 0.25 mM ZnCl2, 98% inhibition of paenidase I and II | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
succinyl-D-Asp-4-methylcoumaryl-7-amide | pH 8, 37°C, paenidase II | Paenibacillus sp. | |
1.03 | - |
succinyl-D-Asp-p-nitroanilide | pH 8, 37°C, paenidase I | Paenibacillus sp. | |
1.25 | - |
succinyl-D-Asp-4-methylcoumaryl-7-amide | pH 8, 37°C, paenidase I | Paenibacillus sp. | |
1.26 | - |
succinyl-D-Asp-p-nitroanilide | pH 8, 37°C, paenidase II | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 33000, paenidase II lacks 5 of the residues present in the N-terminal sequence of paenidase I, paenidase II | Paenibacillus sp. |
34000 | - |
1 * 34000, paenidase I | Paenibacillus sp. |
34170 | - |
paenidase II, paenidase II lacks 5 of the residues present in the N-terminal sequence of paenidase I, MALDI-TOF/MS | Paenibacillus sp. |
34790 | - |
paenidase I, MALDI-TOF/MS | Paenibacillus sp. |
35000 | - |
gel filtration | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
paenidases I and II | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2370 | - |
paenidase II | Paenibacillus sp. |
2880 | - |
paenidase I | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DAEFRH-(D-Asp)-GSY + H2O | synthetic Abeta peptide. No cleavage of the D-Aspcontaining Abeta peptide, paenidase I and II. Paenidases specifically recognize internal D-Asp residues and hydrolyze them on the COOH side | Paenibacillus sp. | DAEFRH-(D-Asp) + GSY | - |
? | |
additional information | no activity with: acetyl-L-Asp-p-nitroanilide, D-Asp-p-nitroanilide, A-Ala-p-nitroanilide, D-Leu-p-nitroanilide, D-Phe-p-nitroanilide, L-Asp-p-nitroanilide, L-Ala-p-nitroanilide, L-Leu-p-nitroanilide, L-Phe-p-nitroanilide, L-Arg-p-nitroanilide, L-Glu-p-nitroanilide, Gly-p-nitroanilide, L-His-p-nitroanilide, L-Ile-p-nitroanilide, L-Lys-p-nitroanilide, L-Met-p-nitroanilide, L-Pro-p-nitroanilide, L-Val-p-nitroanilide, p-nitroanilide, succinyl-L-Ala-L-Pro-L-Ala, succinyl-L-Ala-L-Ala-L-Ala-p-nitroanilide, L-Pyr-p-nitroanilide, Arg-4-methylcoumaryl-7-amide, benzoyl-Arg-4-methylcoumaryl-7-amide, tert-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumaryl-7-amide, Pro-Phe-Arg-4-methylcoumaryl-7-amide, acetyl-Tyr-val-Ala-Asp-4-methylcoumaryl-7-amide, acetyl-ASp-GLu-Val-Asp-4-methylcoumaryl-7-amide, acetyl-Val-Glu-Ile-Asp-4-methylcoumaryl-7-amide | Paenibacillus sp. | ? | - |
? | |
succinyl-D-Asp-4-methylcoumaryl-7-amide + H2O | paenidases specifically recognize internal D-Asp residues and hydrolyze them on the COOH side | Paenibacillus sp. | succinyl-D-Asp + 7-amino-4-methylcoumarin | - |
? | |
succinyl-D-Asp-p-nitroanilide + H2O | paenidases specifically recognize internal D-Asp residues and hydrolyze them on the COOH side | Paenibacillus sp. | succinyl-D-Asp + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, paenidase II lacks 5 of the residues present in the N-terminal sequence of paenidase I, paenidase II | Paenibacillus sp. |
monomer | 1 * 34000, paenidase I | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
DAEP | - |
Paenibacillus sp. |
paenidase | - |
Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 8, 30 min, stable below, paenidases I and II | Paenibacillus sp. |
40 | - |
pH 8, 30 min, paenidase I: about 20% loss of activity, paenidase II: about 30% loss of activity | Paenibacillus sp. |
50 | - |
pH 8, 30 min, about 40% loss of activity, paenidases I and II | Paenibacillus sp. |
60 | - |
pH 8, 30 min, paenidase I: about 80% loss of activity, paenidase II: about 90% loss of activity | Paenibacillus sp. |
70 | - |
pH 8, 30 min, complete loss of activity, paenidases I and II | Paenibacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.66 | - |
succinyl-D-Asp-4-methylcoumaryl-7-amide | pH 8, 37°C, paenidase II | Paenibacillus sp. | |
1.93 | - |
succinyl-D-Asp-4-methylcoumaryl-7-amide | pH 8, 37°C, paenidase I | Paenibacillus sp. | |
4.7 | - |
succinyl-D-Asp-p-nitroanilide | pH 8, 37°C, paenidase I | Paenibacillus sp. | |
6.6 | - |
succinyl-D-Asp-p-nitroanilide | pH 8, 37°C, paenidase II | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
paenidases I and II, succinyl-D-Asp-4-methylcoumaryl-7-amide or succinyl-D-Asp-p-nitroanilide as a substrate | Paenibacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pH 7: about 80% of maximal activity, pH 9.0: about 60% of maximal activity, paenidases I and II, succinyl-D-Asp-4-methylcoumaryl-7-amide as substrate | Paenibacillus sp. |