Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | slightly enhances activity | Neonectria obtusispora | |
EDTA | enhances activity | Neonectria obtusispora | |
GSH | enhances activity | Neonectria obtusispora | |
L-Cys | enhances activity | Neonectria obtusispora | |
L-His | enhances activity | Neonectria obtusispora |
General Stability | Organism |
---|---|
stable to repeated freezing and thawing | Neonectria obtusispora |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Neonectria obtusispora | |
D-Asn | - |
Neonectria obtusispora | |
Fe2+ | - |
Neonectria obtusispora | |
Hg2+ | - |
Neonectria obtusispora | |
iodoacetamide | - |
Neonectria obtusispora | |
Ni2+ | - |
Neonectria obtusispora | |
PCMB | - |
Neonectria obtusispora | |
SDS | - |
Neonectria obtusispora | |
Triton X-100 | - |
Neonectria obtusispora | |
Urea | - |
Neonectria obtusispora | |
Zn2+ | slight inhibition | Neonectria obtusispora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-asparagine | - |
Neonectria obtusispora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CN- | enhances activity | Neonectria obtusispora | |
I- | enhances activity | Neonectria obtusispora | |
additional information | enzyme is not a metalloprotein | Neonectria obtusispora | |
PO43- | enhances activity | Neonectria obtusispora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
216000 | - |
gel filtration | Neonectria obtusispora |
543000 | - |
4 * 543000, SDS-PAGE | Neonectria obtusispora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asn + H2O | Neonectria obtusispora | constitutive enzyme | L-Asp + NH3 | - |
? | |
L-Asn + H2O | Neonectria obtusispora MB-20 | constitutive enzyme | L-Asp + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neonectria obtusispora | - |
MB-20 | - |
Neonectria obtusispora MB-20 | - |
MB-20 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 37.3% w/w carbohydrate | Neonectria obtusispora |
Purification (Comment) | Organism |
---|---|
- |
Neonectria obtusispora |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Neonectria obtusispora |
Storage Stability | Organism |
---|---|
-20°C, stable | Neonectria obtusispora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Asn + H2O | no activity | Neonectria obtusispora | D-Asp + NH3 | - |
? | |
D-Asn + H2O | no activity | Neonectria obtusispora MB-20 | D-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Neonectria obtusispora | L-Asp + NH3 | - |
? | |
L-Asn + H2O | constitutive enzyme | Neonectria obtusispora | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Neonectria obtusispora MB-20 | L-Asp + NH3 | - |
? | |
L-Asn + H2O | constitutive enzyme | Neonectria obtusispora MB-20 | L-Asp + NH3 | - |
? | |
L-Gln + H2O | no activity | Neonectria obtusispora | L-Glu + NH3 | - |
? | |
L-Gln + H2O | no activity | Neonectria obtusispora MB-20 | L-Glu + NH3 | - |
? | |
poly-L-asparagine + H2O | at 21.7% of the activity with L-Asn | Neonectria obtusispora | ? | - |
? | |
poly-L-asparagine + H2O | at 21.7% of the activity with L-Asn | Neonectria obtusispora MB-20 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 543000, SDS-PAGE | Neonectria obtusispora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Neonectria obtusispora |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 42 | 20°C: about 30% of maximal activity, 42°C: about 35% of maximal activity | Neonectria obtusispora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
30 min, stable | Neonectria obtusispora |
40 | - |
30 min, 20% loss of activity | Neonectria obtusispora |
50 | - |
30 min, complete loss of activity | Neonectria obtusispora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Neonectria obtusispora |