Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Tween-20 | Nocardiopsis alba | |
potassium iodide | 231.68% activity at 1 mM | Nocardiopsis alba | |
Triton X-100 | 161.26% activity at 1% (v/v) | Nocardiopsis alba | |
Tween-80 | 123.45% activity at 1% (v/v) | Nocardiopsis alba |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Nocardiopsis alba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | complete inhibition at 2 mM | Nocardiopsis alba | |
Ca2+ | 23.69% residual activity at 1 mM | Nocardiopsis alba | |
Cd2+ | complete inhibition at 1 mM | Nocardiopsis alba | |
Co2+ | 91.24% residual activity at 1 mM | Nocardiopsis alba | |
Cu2+ | complete inhibition at 1 mM | Nocardiopsis alba | |
cysteine | complete inhibition at 1 mM | Nocardiopsis alba | |
Hg2+ | complete inhibition at 1 mM | Nocardiopsis alba | |
iodoacetamide | complete inhibition at 1 mM | Nocardiopsis alba | |
Mg2+ | 29.47% residual activity at 1 mM | Nocardiopsis alba | |
Mn2+ | 31.56% residual activity at 1 mM | Nocardiopsis alba | |
additional information | urea and EDTA do not reveal any inhibitory effects on enzyme activity | Nocardiopsis alba | |
SDS | 33.21% residual activity at 2% (w/v) | Nocardiopsis alba | |
Zn2+ | 10.43% residual activity at 1 mM | Nocardiopsis alba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.127 | - |
L-asparagine | at pH 8.0 and 37°C | Nocardiopsis alba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 145.97% activity at 1 mM | Nocardiopsis alba | |
K+ | 123.41% activity at 1 mM | Nocardiopsis alba | |
Na+ | 128.21% activity at 1 mM | Nocardiopsis alba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Nocardiopsis alba | highly specific substrate | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Nocardiopsis alba NIOT-VKMA08 | highly specific substrate | L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardiopsis alba | W0KM71 | - |
- |
Nocardiopsis alba NIOT-VKMA08 | W0KM71 | - |
- |
Purification (Comment) | Organism |
---|---|
partially purified by ammonium sulfate precipitation and Q-Sepharose column chromatography | Nocardiopsis alba |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
crude enzyme, at pH 8.0 and 37°C | Nocardiopsis alba |
93.28 | - |
after 58.44fold purification, at pH 8.0 and 37°C | Nocardiopsis alba |
Storage Stability | Organism |
---|---|
-20°C, 3 weeks, lyophilized enzyme, no loss of activity | Nocardiopsis alba |
22°C, 24 h, lyophilized enzyme, no loss of activity | Nocardiopsis alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | highly specific substrate | Nocardiopsis alba | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | highly specific substrate | Nocardiopsis alba NIOT-VKMA08 | L-aspartate + NH3 | - |
? | |
additional information | no activity with L-glutamine, L-aspartic acid, L-glutamic acid, thiourea, L-histidine, glutathione, L-arginine, and glycine | Nocardiopsis alba | ? | - |
? | |
additional information | no activity with L-glutamine, L-aspartic acid, L-glutamic acid, thiourea, L-histidine, glutathione, L-arginine, and glycine | Nocardiopsis alba NIOT-VKMA08 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AnsA | - |
Nocardiopsis alba |
L-asparaginase | - |
Nocardiopsis alba |
L-asparagine amidohydrolase | - |
Nocardiopsis alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Nocardiopsis alba |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | the enzyme is active between 20 and 75°C. At temperatures above 37°C, reaction rate declines sharply with the loss of 80% of its original activity at 75°C | Nocardiopsis alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Nocardiopsis alba |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | the enzyme is active between pH 6.5 and 10.0, while at lower pH, enzyme activity is decreasing (20%). There is more than 80% of maximum activity between pH 7.0 and 9.0 | Nocardiopsis alba |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | the enzyme preserves more than 90% of its original activity when incubated up to 36 h at pH 8.0-10.0 | Nocardiopsis alba |