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Literature summary for 3.5.1.1 extracted from
- L-Asparaginase from Aspergillus spp. production based on kinetics, thermal stability and biochemical characterization (2019), 3 Biotech, 9, 289 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM | Aspergillus niger |  |
| Cu2+ | 10 mM | Aspergillus oryzae | |
| iodoacetamide | - |
Aspergillus niger | |
| iodoacetamide | - |
Aspergillus oryzae | |
| additional information | not inhibitory: high NaCl conditions | Aspergillus niger | |
| additional information | not inhibitory: high NaCl conditions | Aspergillus oryzae | |
| p-chloromercuribenzoate | - |
Aspergillus niger | |
| p-chloromercuribenzoate | - |
Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.41 | - |
L-asparagine | pH 8.0, 40°C | Aspergillus niger | |
| 2.1 | - |
L-asparagine | pH 8.0, 40°C | Aspergillus oryzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activation | Aspergillus niger | |
| Mn2+ | activation | Aspergillus oryzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger LBA 02 | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Aspergillus oryzae CCTZ 3940 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Aspergillus niger | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Aspergillus oryzae | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Aspergillus oryzae CCTZ 3940 | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Aspergillus niger LBA 02 | L-aspartate + NH3 | - |
? | |
| additional information | L-glutamine is a poor substrate | Aspergillus niger | ? | - |
? | |
| additional information | no substrate: L-glutamine | Aspergillus oryzae | ? | - |
? | |
| additional information | no substrate: L-glutamine | Aspergillus oryzae CCTZ 3940 | ? | - |
? | |
| additional information | L-glutamine is a poor substrate | Aspergillus niger LBA 02 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | - |
Aspergillus niger |
| 40 | 50 | - |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for 60 min | Aspergillus niger |
| 50 | - |
stable for 60 min | Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Aspergillus niger |
| 5 | 8 | - |
Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Aspergillus niger |
| 5 | 8 | - |
Aspergillus oryzae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | comparison of native L-asparaginases from Aspergillus strains | Aspergillus niger |
| physiological function | comparison of native L-asparaginases from Aspergillus strains | Aspergillus oryzae |
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