Any feedback?
Literature summary for 3.5.1.1 extracted from
- Expression and characterization of recombinant L-asparaginase from Pseudomonas fluorescens (2018), Protein Expr. Purif., 143, 83-91 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 10% inhibition | Pseudomonas fluorescens |  |
| Co2+ | 1 mM, 53 inhibition | Pseudomonas fluorescens | |
| Cu2+ | 1 mM, 47inhibition | Pseudomonas fluorescens | |
| cysteine | 1 mM, 13% inhibition | Pseudomonas fluorescens | |
| EDTA | 1 mM, 19% inhibition | Pseudomonas fluorescens | |
| iodoacetamide | 1 mM, 20% inhibition | Pseudomonas fluorescens | |
| N-bromosuccinimide | 1 mM, 5% inhibition | Pseudomonas fluorescens | |
| Pb2+ | 1 mM, 59inhibition | Pseudomonas fluorescens | |
| PMSF | 1 mM, 63% inhibition | Pseudomonas fluorescens | |
| SDS | 2%, % inhibition | Pseudomonas fluorescens | |
| Sodium azide | 1 mM, 15% inhibition | Pseudomonas fluorescens | |
| Urea | 1 mM, 13% inhibition | Pseudomonas fluorescens | |
| Zn2+ | 1 mM, 54inhibition | Pseudomonas fluorescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM, 149% of initial activity | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
gel filtration | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens MTCC 8127 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
pH 8.0, 37°C | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Pseudomonas fluorescens | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Pseudomonas fluorescens MTCC 8127 | L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas fluorescens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
