Protein Variants | Comment | Organism |
---|---|---|
H100A | soluble, shows enzymic activity | Rattus norvegicus |
H106A | insoluble, no enzymatic activity | Rattus norvegicus |
H106E | insoluble, no enzymatic activity | Rattus norvegicus |
H106K | insoluble, no enzymatic activity | Rattus norvegicus |
H106N | insoluble, no enzymatic activity | Rattus norvegicus |
H106Q | insoluble, no enzymatic activity | Rattus norvegicus |
H106R | insoluble, no enzymatic activity | Rattus norvegicus |
H113A | insoluble, shows enzymic activity | Rattus norvegicus |
H125A | soluble, shows enzymic activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, wild type | Rattus norvegicus | |
0.0083 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, H125A mutant | Rattus norvegicus | |
0.009 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, H113A mutant | Rattus norvegicus | |
0.0095 | - |
10-formyltetrahydrofolate | 30°C, pH 7.8, H100A mutant | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
10-formyltetrahydrofolate + H2O = formate + tetrahydrofolate | dehydrogenase reaction requires full length enzyme, hydrolase reaction occurs in amino-terminal domain of enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + H2O | - |
Rattus norvegicus | formate + tetrahydrofolate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | required for dehydrogenase reaction | Rattus norvegicus |