Literature summary for 3.5.1.106 extracted from

Jimenez, J.; Canales, A.; Jimenez-Barbero, J.; Ginalski, K.; Rychlewski, L.; Garcia, J.; Diaz, E.
Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440 (2008), Proc. Natl. Acad. Sci. USA, 105, 11329-11334.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
D125A	mutation leads to a complete loss of the deformylase activity	Pseudomonas putida
E221A	70% of wild-type deformylase acticity	Pseudomonas putida
H245A	mutation leads to a complete loss of the deformylase activity	Pseudomonas putida
S101A	mutation leads to a complete loss of the deformylase activity	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	x * 29000, SDS-PAGE	Pseudomonas putida
29100	-	x * 29100, calculated from sequence	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-formylmaleamic acid + H2O	Pseudomonas putida	aerobic catabolism of nicotinic acid	maleamate + formate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-
Pseudomonas putida KT 2240	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-formylmaleamic acid + H2O	aerobic catabolism of nicotinic acid	Pseudomonas putida	maleamate + formate	-	?
N-formylmaleamic acid + H2O	S101, D125, and H245 are essential for the enzyme activity, constituting the catalytic triad of the NicD deformylase	Pseudomonas putida	maleamate + formate	-	?

Subunits

Subunits	Comment	Organism
?	x * 29000, SDS-PAGE	Pseudomonas putida
?	x * 29100, calculated from sequence	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
NicD	-	Pseudomonas putida

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Release 2023.1 (January 2023)