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Literature summary for 3.5.1.12 extracted from
- Lipoamidase and biotinidase activities in the rat: Tissue distribution and intracellular localization (1994), Eur. J. Clin. Chem. Clin. Biochem., 32, 501-509.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | - |
Homo sapiens |  |
| CuCl2 | - |
Rattus rattus | |
| diisopropylfluorophosphate | - |
Rattus rattus | |
| Hg2+ | - |
Homo sapiens | |
| Hg2+ | - |
Rattus rattus | |
| p-hydroxymercuribenzoate | - |
Homo sapiens | |
| p-hydroxymercuribenzoate | - |
Rattus rattus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
lipoyl-4-aminobenzoic acid + | pH 9.5 | Rattus rattus | |
| 1.3 | - |
lipoyllysine | - |
Rattus rattus | |
| 1.3 | - |
lipoyl-4-aminobenzoic acid + | pH 6.0 | Rattus rattus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Cavia porcellus | - |
- |
| microsome | - |
Rattus rattus | - |
- |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Rattus rattus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biotin amide + H2O | Cavia porcellus | - |
? | - |
? | |
| biotin amide + H2O | Homo sapiens | - |
? | - |
? | |
| biotin amide + H2O | Rattus rattus | - |
? | - |
? | |
| epsilon-N-(D,L-lipoyl)-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| epsilon-N-(D,L-lipoyl)-L-lysine + H2O | Rattus rattus | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Cavia porcellus | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Rattus rattus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
guinea pig | - |
| Homo sapiens | - |
human | - |
| Rattus rattus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | serum, plasma, leukocytes | Homo sapiens | - |
| blood | serum, plasma, leukocytes | Rattus rattus | - |
| brain | - |
Cavia porcellus | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Rattus rattus | - |
| kidney | - |
Homo sapiens | - |
| kidney | - |
Rattus rattus | - |
| large intestine | - |
Rattus rattus | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus rattus | - |
| lung | - |
Rattus rattus | - |
| pancreas | - |
Rattus rattus | - |
| small intestine | - |
Homo sapiens | - |
| small intestine | - |
Rattus rattus | - |
| spleen | - |
Rattus rattus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (N-D,L-lipoyl-p-aminobenzoic acid) + H2O | - |
Homo sapiens | ? | - |
? | |
| (N-D,L-lipoyl-p-aminobenzoic acid) + H2O | - |
Rattus rattus | ? | - |
? | |
| biotin amide + H2O | - |
Cavia porcellus | ? | - |
? | |
| biotin amide + H2O | - |
Homo sapiens | ? | - |
? | |
| biotin amide + H2O | - |
Rattus rattus | ? | - |
? | |
| epsilon-N-(D,L-lipoyl)-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| epsilon-N-(D,L-lipoyl)-L-lysine + H2O | - |
Rattus rattus | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Cavia porcellus | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Cavia porcellus | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | ? | - |
? | |
| lipoyl-4-aminobenzoic acid + H2O | - |
Rattus rattus | ? | - |
? | |
| lipoyllysine + H2O | - |
Rattus rattus | ? | - |
? | |
| p-(N-biotinylamino)benzoic acid + H2O | - |
Homo sapiens | biotin + p-aminobenzoic acid | - |
ir | |
| p-(N-biotinylamino)benzoic acid + H2O | - |
Rattus rattus | biotin + p-aminobenzoic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipoamidase | biotinidase and lipoamidase can be separated from each other | Cavia porcellus |
| lipoamidase | not yet given an EC number, seems to be identical with biotinidase | Rattus rattus |
| lipolysine hydrolase | not yet given an EC number, seems to be identical with biotinidase | Rattus rattus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | - |
Homo sapiens |
| 60 | 70 | - |
Rattus rattus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
lipoyllysine as substrate | Rattus rattus |
| 6 | - |
enzyme from plasma, liver enzyme has 2 pH optima at pH 6.0 and pH 9.5 with lipoyl-p-aminobenzoic acid as substrate | Rattus rattus |
| 9.5 | - |
liver enzyme has 2 pH optima at pH 6.0 and pH 9.5 with lipoyl-p-aminobenzoic acid as substrate | Rattus rattus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | - |
Rattus rattus |
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