General Stability | Organism |
---|---|
significant loss of activity upon freezing at -20°C and -80°C in presence and absence of 20% glycerol | Escherichia coli |
sodium borate is the most effective stabilizing agent against cold inactivation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
glutaminase B, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutaminase B | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, in presence of sodium borate, stable for 5 days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gln + H2O | - |
Escherichia coli | Glu + NH3 | - |
? | |
L-Gln + hydroxylamine | - |
Escherichia coli | L-gamma-glutamyl hydroxamate + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
low level of activity after partial purification at 4°C in absence of stabilizing ligands, warming at 24°C results in 2fold to 5fold increase in activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | 9 | hydrolysis of Gln, glutaminase B | Escherichia coli |
8.3 | 9.2 | formation of gamma-glutamyl hydroxamate from L-Gln and NH2OH | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | pH 6.0: about 55% of maximal activity, pH 11.0: about 55% of maximal activity, hydrolysis of Gln | Escherichia coli |