Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Micrococcus luteus |
Protein Variants | Comment | Organism |
---|---|---|
E160D | inactive mutant enzyme | Micrococcus luteus |
E160D | mutant enzyme with largely decreased catalytic activity, kcat/Km is 1167fold lower than wild-type value | Micrococcus luteus |
K67E | inactive mutant enzyme | Micrococcus luteus |
S64A | inactive mutant enzyme | Micrococcus luteus |
Y191F | kcat/Km is 39fold lower than wild-type value | Micrococcus luteus |
Y191T | kcat/Km is 67742fold lower than wild-type value | Micrococcus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Micrococcus luteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.2 | - |
L-glutamine | mutant enzyme E160D | Micrococcus luteus | |
6.6 | - |
L-glutamine | wild-type enzyme | Micrococcus luteus | |
11 | - |
L-glutamine | mutant enzyme Y191F | Micrococcus luteus | |
460 | - |
L-glutamine | mutant enzyme Y191T | Micrococcus luteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Micrococcus luteus K-3 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Micrococcus luteus | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Micrococcus luteus K-3 | L-glutamate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
L-glutamine | mutant enzyme E160D | Micrococcus luteus | |
1.4 | - |
L-glutamine | mutant enzyme Y191T | Micrococcus luteus | |
57 | - |
L-glutamine | mutant enzyme Y191F | Micrococcus luteus | |
1400 | - |
L-glutamine | wild-type enzyme | Micrococcus luteus |