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Literature summary for 3.5.1.2 extracted from

  • Brown, G.; Singer, A.; Proudfoot, M.; Skarina, T.; Kim, Y.; Chang, C.; Dementieva, I.; Kuznetsova, E.; Gonzalez, C.F.; Joachimiak, A.; Savchenko, A.; Yakunin, A.F.
    Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis (2008), Biochemistry, 47, 5724-5735.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
-
 Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli
-
 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
G261A 54,7% wild-type activity, Km: 23.7 mM Escherichia coli
Q162A wild-type level of activity Escherichia coli
S260A 16.5% of wild-type activity Escherichia coli
S66A very low activity Escherichia coli
Y29A 32.5% of wild-type activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine
-
 Bacillus subtilis
6-diazo-5-oxo-L-norleucine
-
 Escherichia coli
ampicillin
-
 Escherichia coli
Mg2+
-
 Bacillus subtilis
Mg2+
-
 Escherichia coli
Mn2+
-
 Bacillus subtilis
Mn2+
-
 Escherichia coli
penicillin
-
 Escherichia coli
PO43-
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3
-
 L-glutamine
-
 Escherichia coli
7.6
-
 L-glutamine
-
 Bacillus subtilis
8.5
-
 L-glutamine mutant Q162A Escherichia coli
23.7
-
 L-glutamine mutant G261A Escherichia coli
27.6
-
 L-glutamine
-
 Bacillus subtilis
30.6
-
 L-glutamine
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32900
-
 4 * 32900, SDS-PAGE Escherichia coli
33500
-
 2 * 33500, SDS-PAGE Escherichia coli
34000
-
 2 * 34000, SDS-PAGE Bacillus subtilis
36200
-
 4 * 36200, SDS-PAGE, gelfiltration Bacillus subtilis
72600
-
 gel filtration Bacillus subtilis
74900
-
 gel filtration Escherichia coli
138100
-
 gel filtration Bacillus subtilis
148800
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O07637 ATCC 23857D-5
-
Bacillus subtilis O31465 ATCC 23857D-5
-
Bacillus subtilis 168 O07637 ATCC 23857D-5
-
Bacillus subtilis 168 O31465 ATCC 23857D-5
-
Escherichia coli P0A6W0
-
-
Escherichia coli P77454
-
-
Escherichia coli W3110 / ATCC 27325 P0A6W0
-
-
Escherichia coli W3110 / ATCC 27325 P77454
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
stable at -196°C (unstable at -80°C) Escherichia coli
stable at -80?C Bacillus subtilis
stable at -80°C Bacillus subtilis
stable at -80°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate-p-nitroanilide + H2O
-
 Escherichia coli L-glutamate + 4-nitroaniline
-
 ?
L-glutamate-p-nitroanilide + H2O
-
 Bacillus subtilis L-glutamate + 4-nitroaniline
-
 ?
L-glutamate-p-nitroanilide + H2O
-
 Bacillus subtilis 168 L-glutamate + 4-nitroaniline
-
 ?
L-glutamate-p-nitroanilide + H2O
-
 Escherichia coli W3110 / ATCC 27325 L-glutamate + 4-nitroaniline
-
 ?
L-glutamine + H2O
-
 Escherichia coli L-glutamic acid + NH3
-
 ?
L-glutamine + H2O
-
 Bacillus subtilis L-glutamic acid + NH3
-
 ?
L-glutamine + H2O
-
 Bacillus subtilis 168 L-glutamic acid + NH3
-
 ?
L-glutamine + H2O
-
 Escherichia coli W3110 / ATCC 27325 L-glutamic acid + NH3
-
 ?
nitrocefin + H2O
-
 Escherichia coli (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Escherichia coli W3110 / ATCC 27325 (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 34000, SDS-PAGE Bacillus subtilis
homodimer 2 * 33500, SDS-PAGE Escherichia coli
homotetramer 4 * 32900, SDS-PAGE Escherichia coli
homotetramer 4 * 36200, SDS-PAGE, gelfiltration Bacillus subtilis

Synonyms

Synonyms Comment Organism
glutaminase
-
 Escherichia coli
glutaminase
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.6
-
 L-glutamine
-
 Bacillus subtilis
49.9
-
 L-glutamine mutant Q161A Escherichia coli
67.7
-
 L-glutamine
-
 Bacillus subtilis
79.9
-
 L-glutamine mutant Q162A Escherichia coli
91.4
-
 L-glutamine
-
 Escherichia coli
101
-
 L-glutamine
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Escherichia coli
7.5 8
-
 Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5
-
 ampicillin competitive inhibitor Escherichia coli
6.4
-
 penicillin
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.1
-
-
 Escherichia coli Mn2+
0.2
-
-
 Escherichia coli Mg2+
0.7 1.3
-
 Bacillus subtilis Mn2+
0.7 1.3
-
 Escherichia coli Mn2+
1.7 5.5
-
 Bacillus subtilis Mg2+
1.7 5.5
-
 Escherichia coli Mg2+
17.2 17.4
-
 Escherichia coli PO43-