Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.2 extracted from

  • Jeon, J.; Lee, H.; So, J.
    Glutaminase activity of Lactobacillus reuteri KCTC3594 and expression of the activity in other Lactobacillus spp. by introduction of the glutaminase gene (2009), Afr. J. Microbiol. Res., 3, 605-609.
No PubMed abstract available
Search for more literature for 3.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Lactobacillus plantarum KLB213 which exhibits a high salt-tolerance and Lactobacillus paracasei KLB58 which produces high amount of exopolysaccharide Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5
-
 L-glutamine pH 7.5, 37°C Limosilactobacillus reuteri

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl enzyme is salt-tolerant as the relative activity displays 110% in the condition of 5% NaCl and remains over 50% in the presence of 20% NaCl Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri
-
 KCTC3594
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the recombinant enzymes from Lactobacillus plantarum and Lactobacillus paracasei show increased enzyme activity. The wild-type organisms do not amplify the glutaminase gene. The recombinant Lactobacillus plantarum displays ca. 4fold higher activity than its wild-type and 1.5fold higher activity the wild-type Lactobacillus reuteri. The recombinant Lactobacillus paracasei enzyme increases to half of the activity of Lactobacillus reuteri. The wild-type Lactobacillus paracasei shows no glutaminase activity Limosilactobacillus reuteri
0.516
-
 pH 7.5, 40°C, Lactobacillus reuteri shows the highest activity compared to wild-type Lactobacillus plantarum and wild-type Lactobacillus paracasei which are transformed with the glutaminase gene Lactobacillus reuteri in this study. The wild-type Lactobacillus paracasei shows no glutaminase activity Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + H2O specifc for L-glutamine Limosilactobacillus reuteri L-glutamate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Limosilactobacillus reuteri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 78% activity remains after incubation at 60°C for 30 min Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Limosilactobacillus reuteri

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 stable at Limosilactobacillus reuteri