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Literature summary for 3.5.1.24 extracted from
- Identification and characterization of a bile salt hydrolase from Lactobacillus salivarius for development of novel alternatives to antibiotic growth promoters (2012), Appl. Environ. Microbiol., 78, 8795-8802.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | enzyme inhibitors are promising feed additives to replace antibiotic growth promoters for enhancing the productivity and sustainability of food animals | Ligilactobacillus salivarius |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) clone (JL 885) | Ligilactobacillus salivarius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 22.4% inhibition at 5 mM | Ligilactobacillus salivarius |  |
| CoCl2 | 95.9% inhibition at 5 mM | Ligilactobacillus salivarius | |
| CuCl2 | 98.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| CuSO4 | 91.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| FeCl3 | 73% inhibition at 5 mM | Ligilactobacillus salivarius | |
| FeSO4 | 96.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| KCl | 25.9% inhibition at 5 mM | Ligilactobacillus salivarius | |
| KI | 36.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
| KIO3 | 96.5% inhibition at 5 mM | Ligilactobacillus salivarius | |
| MgCl2 | 25.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| MgSO4 | 31.3% inhibition at 5 mM | Ligilactobacillus salivarius | |
| MnCl2 | 68.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| MnSO4 | 83.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| additional information | no inhibition by citric acid | Ligilactobacillus salivarius | |
| Na2SeO3 | 93.1% inhibition at 5 mM | Ligilactobacillus salivarius | |
| Na2SO4 | 27.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| NaCl | 27.7% inhibition at 5 mM | Ligilactobacillus salivarius | |
| NaHCO3 | 20.6% inhibition at 5 mM | Ligilactobacillus salivarius | |
| NaI | 31.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
| NaIO3 | 86.4% inhibition at 5 mM | Ligilactobacillus salivarius | |
| NaIO4 | 96.4% inhibition at 5 mM | Ligilactobacillus salivarius | |
| vitamin C | 21.8% inhibition at 5 mM | Ligilactobacillus salivarius | |
| ZnCl2 | 68.3% inhibition at 5 mM | Ligilactobacillus salivarius | |
| ZnSO4 | 89.5% inhibition at 5 mM | Ligilactobacillus salivarius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.15 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 1.71 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 2.48 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 2.5 | 3 | taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.19 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.21 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | Ligilactobacillus salivarius | best substrate | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | best substrate | chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | Ligilactobacillus salivarius | - |
cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
deoxycholate + glycine | - |
? | |
| taurochenodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | Ligilactobacillus salivarius | - |
cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Ligilactobacillus salivarius | - |
deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Ligilactobacillus salivarius NRRL B-30514 | - |
deoxycholate + taurine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ligilactobacillus salivarius | J7H3P9 | isolated from chicken | - |
| Ligilactobacillus salivarius NRRL B-30514 | J7H3P9 | isolated from chicken | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme from Escherichia coli strain BL21(DE3) clone (JL 885) by nickel affinity chromatography | Ligilactobacillus salivarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycodeoxycholic acid | Ligilactobacillus salivarius |
| 5.6 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurocholic acid | Ligilactobacillus salivarius |
| 7.7 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycocholic acid | Ligilactobacillus salivarius |
| 8 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurochenodeoxycholic acid | Ligilactobacillus salivarius |
| 8.5 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate taurodeoxycholic acid | Ligilactobacillus salivarius |
| 17.7 | - |
pH 5.4, 37°C, purified recombinant enzyme, substrate glycochenodeoxycholic acid | Ligilactobacillus salivarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | best substrate | Ligilactobacillus salivarius | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | best substrate | Ligilactobacillus salivarius NRRL B-30514 | chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | deoxycholate + glycine | - |
? | |
| additional information | broad substrate specificity | Ligilactobacillus salivarius | ? | - |
? | |
| additional information | broad substrate specificity | Ligilactobacillus salivarius NRRL B-30514 | ? | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Ligilactobacillus salivarius | cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius | deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Ligilactobacillus salivarius NRRL B-30514 | deoxycholate + taurine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Ligilactobacillus salivarius |
| BSH | - |
Ligilactobacillus salivarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41 | - |
recombinant enzyme | Ligilactobacillus salivarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 55 | activity range, profile overview | Ligilactobacillus salivarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.37 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 8.5 | - |
taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 8.87 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 9.75 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 13.43 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 15.6 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
recombinant enzyme | Ligilactobacillus salivarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | activity range, profile overview | Ligilactobacillus salivarius |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.04 | - |
taurocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 3.36 | - |
taurochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 4.21 | - |
taurodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 5.19 | - |
glycocholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 5.54 | - |
glycodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
| 6.29 | - |
glycochenodeoxycholic acid | pH 5.4, 37°C, recombinant enzyme | Ligilactobacillus salivarius | |
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