Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithio-bis(2-nitrobenzoate) | - |
Escherichia coli | |
acetate | competitive | Escherichia coli | |
N-acetyl-D-glucosamine 6-phosphate | substrate inhibition at high concentrations | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
N-acetyl-D-glucosamine 6-phosphate | - |
Escherichia coli | |
20 | - |
acetate | - |
Escherichia coli | |
25 | - |
glucosamine 6-phosphate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, SDS-PAGE | Escherichia coli |
160000 | 165000 | non-denaturing PAGE, gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overproducing strain | - |
Escherichia coli overproducing | - |
overproducing strain | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate | sequential mechanism with ordered release of products and a slow isomerization of the enzyme-acetate complex | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 6-phosphate + acetate | - |
Escherichia coli | N-acetyl-D-glucosamine 6-phosphate + H2O | - |
r | |
D-glucosamine 6-phosphate + acetate | - |
Escherichia coli overproducing | N-acetyl-D-glucosamine 6-phosphate + H2O | - |
r | |
N-acetyl-D-glucosamine 6-phosphate + H2O | - |
Escherichia coli | D-glucosamine 6-phosphate + acetate | - |
r | |
N-acetyl-D-glucosamine 6-phosphate + H2O | - |
Escherichia coli overproducing | D-glucosamine 6-phosphate + acetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
105 | - |
N-acetyl-D-glucosamine 6-phosphate | - |
Escherichia coli |