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Literature summary for 3.5.1.3 extracted from
- Rat liver omega-amidase. Kinetic evidence for an acyl-enzyme intermediate (1972), Biochemistry, 11, 2251-2256.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | uncompetitive activation | Rattus norvegicus |  |
| methanol | uncompetitive activation | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2,2-Trifluoroethanol | more than 2 M: irreversible inactivation | Rattus norvegicus | |
| 2-Chloroethanol | more than 2 M: irreversible inactivation | Rattus norvegicus | |
| dioxane | up to 5% dioxane: no effect on hydrolysis of ethyl alpha-keto-glutarate or glutaramate, at 10% dioxane: slight, 5-10% inhibition | Rattus norvegicus | |
| ethanol | more than 2 M: irreversible inactivation | Rattus norvegicus | |
| methylamine | competitve inhibition, inhibits hydroxaminolysis of glutaramate and alpha-ketoglutaramate hydrolysis | Rattus norvegicus | |
| additional information | no inhibition by ethylamine | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic studies, criteria and data | Rattus norvegicus | |
| 1 | - |
hydroxylamine | with substrate, acyl donor: p-methylphenyl glutarate | Rattus norvegicus | |
| 2 | - |
Glutaramate | hydrolysis | Rattus norvegicus | |
| 2 | - |
p-methylphenyl glutarate | hydrolysis | Rattus norvegicus | |
| 3 | - |
methyl glutarate | hydrolysis | Rattus norvegicus | |
| 3.7 | - |
p-chlorophenyl glutarate | hydrolysis | Rattus norvegicus | |
| 3.7 | - |
hydroxylamine | with substrate, acyl donor: glutaramate | Rattus norvegicus | |
| 4.1 | - |
hydroxylamine | with substrate, acyl donor: methyl glutarate | Rattus norvegicus | |
| 8 | - |
p-methoxyphenyl glutarate | hydrolysis | Rattus norvegicus | |
| 8 | - |
phenyl glutarate | hydrolysis | Rattus norvegicus | |
| 30 | - |
methylamine | with substrate, acyl donor: methyl glutarate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller | Rattus norvegicus | |
| 43 | - |
methylamine | with substrate, acyl donor: glutaramate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller | Rattus norvegicus | |
| 55 | - |
methylamine | with substrate, acyl donor: p-methylphenyl glutarate, Km for total methylamine, for free-base form of methylamine, Km is about 102 times smaller | Rattus norvegicus | |
| 5000 | - |
methanol | with substrate, acyl donor: glutaramate | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutaramate + H2O | Rattus norvegicus | alpha-ketoglutaramate | 2-oxoglutarate + NH3 | - |
? | |
| additional information | Rattus norvegicus | enzyme, in conjunction with glutamine transaminase comprises enzyme system previously designated glutaminase II, involved in metabolism of glutamine | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2,2,2-trifluoroethanol | more than 2 M: unstable to | Rattus norvegicus |
| 2-chloroethanol | more than 2 M: unstable to | Rattus norvegicus |
| Ethanol | more than 2 M: unstable to | Rattus norvegicus |
| Methanol | more than 2 M: stable to and uncompetitive activation | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | kinetic evidence for an acyl-enzyme intermediate | Rattus norvegicus | |
| a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | two-step mechanism | Rattus norvegicus | |
| a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | acyl-enzyme mechanism | Rattus norvegicus | |
| a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | catalytic sequence involves two steps and an acyl-enzyme intermediate | Rattus norvegicus | |
| a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 | double displacement or ping-pong mechanism | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.4 | - |
ethyl alpha-ketoglutarate hydrolysis | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutaramate + H2O | alpha-ketoglutaramate | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? | |
| delta-ethyl 2-oxoglutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| gamma-ethyl 2-oxoglutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| glutaramate + H2O | - |
Rattus norvegicus | glutarate + NH3 | - |
? | |
| glutaramate + hydroxylamine | - |
Rattus norvegicus | glutaryl hydroxamate + NH3 | - |
? | |
| glutaramate + methanol | methanolysis | Rattus norvegicus | glutarate methyl ester + hydroxylamine | - |
? | |
| glutaramate + methylamine | - |
Rattus norvegicus | ? | - |
? | |
| glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| glutarate + hydroxylamine | acyl-transfer reaction | Rattus norvegicus | glutaryl hydroxamate | - |
? | |
| methyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| methyl glutarate + hydoxylamine | - |
Rattus norvegicus | glutaramate + methanol | - |
? | |
| methyl glutarate + methylamine | - |
Rattus norvegicus | ? | - |
? | |
| additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
| additional information | catalyzes hydrolysis and acyl-transfer reactions with amide and monoalkyl esters of alpha-ketoglutarate, glutarate, and succinate | Rattus norvegicus | ? | - |
? | |
| additional information | catalyzes methanolysis, no alcoholysis using ethanol, 2-chloroethanol, and 2,2,2-trifluoroethanol | Rattus norvegicus | ? | - |
? | |
| additional information | rate of hydrolysis is decreased proportionally to the increase in hydroxaminolysis | Rattus norvegicus | ? | - |
? | |
| additional information | catalyzes hydroxaminolysis and transamidation reactions with esters and amides | Rattus norvegicus | ? | - |
? | |
| additional information | catalyzes hydrolysis and hydroxaminolysis | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme, in conjunction with glutamine transaminase comprises enzyme system previously designated glutaminase II, involved in metabolism of glutamine | Rattus norvegicus | ? | - |
? | |
| p-chlorophenyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| p-methoxyphenyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| p-methylphenyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| p-methylphenyl glutarate + hydroxylamine | - |
Rattus norvegicus | glutaramate + 4-methylphenol | - |
? | |
| p-methylphenyl glutarate + methylamine | - |
Rattus norvegicus | ? | - |
? | |
| phenyl glutarate + H2O | - |
Rattus norvegicus | ? | - |
? | |
| succinate + H2O | - |
Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
p-methylphenyl glutarate hydrolysis and ethyl alpha-ketoglutarate hydrolysis, assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
p-methylphenyl glutarate hydrolysis, assay at | Rattus norvegicus |
| 7.4 | - |
ethyl alpha-keto-glutarate hydrolysis, assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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