Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxy-4-methoxy-benzophenone | with 1 micromol 2-hydroxy-4-methoxy-benzophenone the activity is 0% after 10 min | Pseudomonas sp. | |
DTNB | with 20 micromol DTNB the activity is 5% after 10 min | Pseudomonas sp. | |
iodoacetamide | with 20 micromol iodoacetamide the activity is 20% after 10 min | Pseudomonas sp. | |
iodoacetate | with 20 micromol iodoacetate the activity is 33% after 10 min | Pseudomonas sp. | |
N-Methylmaleimide | with 20 micromol N-methylmaleimide the activity is 2% after 10 min | Pseudomonas sp. | |
NEM | with 20 micromol NEM the activity is 0% after 10 min | Pseudomonas sp. | |
oxalacetate | a competitive inhibitor | Pseudomonas sp. | |
p-hydroxymercurisulfonate | with 1 micromol p-hydroxymercurisulfonate the activity is 0% after 10 min | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
alpha-ketoglutaramate | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ketoglutaramate + H2O | Pseudomonas sp. | - |
alpha-ketoglutarate + NH3 | - |
? | |
alpha-ketoglutaramate + H2O | Pseudomonas sp. MA / M.A. / ATCC 23819 | - |
alpha-ketoglutarate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. MA / M.A. / ATCC 23819 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
extraction, addition of streptomycin sulfate, adjust the pH value, centrifugation, ammonium sulfate precipitation, dialysis, purification with DEAE, Sephadex and hydroxyapatite column with dialysis and ultrafiltration steps | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
after purification | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 20 mM potassium phosphate buffer, pH 7.4, containing 5 mM alpha-ketoglutarate, six months. In the absence of alpha-ketoglutarate the enzyme slowly loses activity over a period of several weeks | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ketoglutaramate + H2O | - |
Pseudomonas sp. | alpha-ketoglutarate + NH3 | - |
? | |
alpha-ketoglutaramate + H2O | - |
Pseudomonas sp. MA / M.A. / ATCC 23819 | alpha-ketoglutarate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0178 | - |
alpha-ketoglutarate | turnover number based on the molecular weight of 90000 | Pseudomonas sp. |