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Literature summary for 3.5.1.38 extracted from

  • Roberts, J.
    Purification and properties of a highly potent antitumor glutaminase-asparaginase from Pseudomonas 7A (1976), J. Biol. Chem., 251, 2119-2123.
    View publication on PubMed
Search for more literature for 3.5.1.38

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 inhibition above 10 mM Pseudomonas sp.
5-Diazo-4-oxo-L-norvaline marked inhibition at 0.1 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 L-asparagine
-
 Pseudomonas sp.
0.005
-
 L-glutamine
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Pseudomonas sp.
-
 L-aspartate + NH3
-
 ?
L-asparagine + H2O Pseudomonas sp. 7A
-
 L-aspartate + NH3
-
 ?
L-glutamine + H2O Pseudomonas sp.
-
 L-glutamate + NH3
-
 ?
L-glutamine + H2O Pseudomonas sp. 7A
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 7A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to crystalline form, 3step chromatography Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture induction of enzyme synthesis by addition of glutamic acid to the medium, glucose inhibits enzyme synthesis Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyanoalanine + H2O slow hydrolysis rate Pseudomonas sp. alanine + NH3
-
 ?
D-asparagine + H2O
-
 Pseudomonas sp. D-aspartate + NH3
-
 ?
D-asparagine + H2O
-
 Pseudomonas sp. 7A D-aspartate + NH3
-
 ?
D-glutamine + H2O
-
 Pseudomonas sp. D-glutamate + NH3
-
 ?
D-glutamine + H2O
-
 Pseudomonas sp. 7A D-glutamate + NH3
-
 ?
gamma-L-glutamyl hydroxylammonium sulfate + H2O same rate as for L-glutamine Pseudomonas sp. L-glutamate + hydroxylammonium sulfate
-
 ?
L-asparagine + H2O
-
 Pseudomonas sp. L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Pseudomonas sp. 7A L-aspartate + NH3
-
 ?
L-glutamine + H2O
-
 Pseudomonas sp. L-glutamate + NH3
-
 ?
L-glutamine + H2O higher activity for glutaminase than for asparaginase Pseudomonas sp. L-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Pseudomonas sp. 7A L-glutamate + NH3
-
 ?
L-glutamine + H2O higher activity for glutaminase than for asparaginase Pseudomonas sp. 7A L-glutamate + NH3
-
 ?
succinamic acid + H2O slow hydrolysis rate Pseudomonas sp. succinate + NH3
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Pseudomonas sp.