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Literature summary for 3.5.1.38 extracted from
- Kinetic properties of Streptomyces canarius L-glutaminase and its anticancer efficiency (2015), Braz. J. Microbiol., 46, 957-968 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | L-glutaminase has a significant efficiency against Hep-G2 cell (IC50 6.8x02g/ml) and HeLa cells (IC50 8.3 g/ml), while the growth of MCF-7 cells is not affected. L-glutaminase has a moderate cytotoxic effect against HCT-116 cells (IC50 64.7 g/ml) and RAW 264.7 cells (IC50 59.3 g/ml). In vivo, L-glutaminase shows no observed changes in liver, kidney functions, hematological parameters and slight effect on red blood cells and level of platelets after 10 days of rabbit's injection | Streptomyces canarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.129 | - |
L-glutamine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius |  |
| 0.137 | - |
L-asparagine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
| 5.38 | - |
L-aspartate | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces canarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces canarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 132.2 | - |
pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Streptomyces canarius | L-aspartate + NH3 | - |
r | |
| L-aspartate + NH3 | - |
Streptomyces canarius | L-asparagine + H2O | - |
r | |
| L-glutamine + H2O | - |
Streptomyces canarius | L-glutamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44000, SDS-PAGE | Streptomyces canarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Streptomyces canarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57.5 | - |
melting temperature | Streptomyces canarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
L-aspartate | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
| 0.00068 | - |
L-asparagine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
| 0.00075 | - |
L-glutamine | pH not specified in the publication, temperature not specified in the publication | Streptomyces canarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces canarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | - |
Streptomyces canarius |
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Release 2023.1 (January 2023)
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