Any feedback?
Literature summary for 3.5.1.4 extracted from
- Characterization of an inducible amidase from Pseudomonas acidovorans AE1 (1975), Eur. J. Biochem., 53, 357-369.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoic acid | as -SH-blocking reagent, substrate 4-nitroacetanilide, incubation 10 min, 10 micromol 4-chloromercuribenzoic acid, residual activity 4.8%, activity restored by addition of 1 mM cysteine, 1 h incubation 67% restored | Delftia acidovorans |  |
| acetanilide | substrate 4-nitroacetanilide, Ki: 14 micromol, competitive inhibition | Delftia acidovorans | |
| Diethyl-4-nitrophenyl phosphate | - |
Delftia acidovorans | |
| diisopropylphosphorofluoridate | - |
Delftia acidovorans | |
| Hg2+ | HgCl2 as -SH-blocking reagent, incubation 10 min, 10 micromol HgCl2, 81% of activity restored by addition of 1 mM cysteine | Delftia acidovorans | |
| iodoacetamide | concentration 0.2 M at pH 8.0 | Delftia acidovorans | |
| o-Nitrophenol | competitive inhibition | Delftia acidovorans | |
| Phenacetin | substrate 4-nitroacetanilide, Ki: 8.9 nM, competitive inhibition, induction of activity for 2-nitrophenylacetate and methylbutyrate | Delftia acidovorans | |
| Phenetidine | uncompetitive inhibition | Delftia acidovorans | |
| physostigmine sulfate | - |
Delftia acidovorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
4-nitrophenylacetate | - |
Delftia acidovorans | |
| 0.014 | - |
acetanilide | - |
Delftia acidovorans | |
| 0.029 | - |
phenylacetate | - |
Delftia acidovorans | |
| 0.042 | - |
2-nitroacetanilide | - |
Delftia acidovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55300 | - |
amino acid composition | Delftia acidovorans |
| 56700 | - |
SDS-PAGE | Delftia acidovorans |
| 57500 | - |
gel filtration | Delftia acidovorans |
| 59100 | - |
reaction of purified enzyme with diethyl-4-nitrophenyl phosphate | Delftia acidovorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitroacetanilide | Delftia acidovorans | - |
2-nitrophenylamine + acetate | - |
? | |
| 2-nitroacetanilide | Delftia acidovorans AE1 | - |
2-nitrophenylamine + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | Delftia acidovorans | - |
4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | Delftia acidovorans AE1 | - |
4-nitrophenol + acetate | - |
? | |
| phenetidine + acetate | Delftia acidovorans | transferase activity | phenacetin + H2O | - |
? | |
| phenetidine + acetate | Delftia acidovorans AE1 | transferase activity | phenacetin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Delftia acidovorans | - |
- |
- |
| Delftia acidovorans AE1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| lysis of crude extract, addition of streptomycin, ammonium sulfate fractionation, desalting by Sephadex-gel filtration, DEAE-Sepharose chromatography, hydroxyapatite column, yield 72% | Delftia acidovorans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a monocarboxylic acid amide + H2O = a monocarboxylate + NH3 | hydrolysis of ester and aromatic amide | Delftia acidovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
substrate acetamide, crude extract | Delftia acidovorans |
| 7.7 | - |
substrate 4-nitroacetanilide, crude extract | Delftia acidovorans |
| 73 | - |
substrate phenacetin, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 121 | - |
substrate 2-nitroacetanilide, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 133 | - |
substrate acetanilide, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 149 | - |
substrate phenylacetate, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
| 181 | - |
substrate 4-nitrophenylacetate, pure enzyme, pH 8.6 and 30°C | Delftia acidovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitroacetanilide | - |
Delftia acidovorans | 2-nitrophenylamine + acetate | - |
? | |
| 2-nitroacetanilide | - |
Delftia acidovorans AE1 | 2-nitrophenylamine + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Delftia acidovorans | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Delftia acidovorans AE1 | 4-nitrophenol + acetate | - |
? | |
| acetanilide + H2O | - |
Delftia acidovorans | acetate + aniline | - |
? | |
| acetanilide + H2O | - |
Delftia acidovorans AE1 | acetate + aniline | - |
? | |
| p-nitrophenylacetamide + H2O | - |
Delftia acidovorans | p-nitrophenylacetic acid + NH3 | - |
? | |
| phenetidine + acetate | transferase activity | Delftia acidovorans | phenacetin + H2O | - |
? | |
| phenetidine + acetate | transferase activity | Delftia acidovorans AE1 | phenacetin + H2O | - |
? | |
| phenylacetamide | - |
Delftia acidovorans | phenylacetic acid + NH3 | - |
? | |
| phenylacetamide | - |
Delftia acidovorans AE1 | phenylacetic acid + NH3 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 129 | - |
2-nitroacetanilide | - |
Delftia acidovorans | |
| 134 | - |
acetanilide | - |
Delftia acidovorans | |
| 144 | - |
4-nitrophenylacetate | - |
Delftia acidovorans | |
| 147 | - |
phenylacetate | - |
Delftia acidovorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Delftia acidovorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Delftia acidovorans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000089 | - |
Phenacetin | substrate 4-nitroacetanilide, competitive inhibition, induction of activity for 2-nitrophenylacetate and methylbutyrate | Delftia acidovorans | |
| 0.0014 | - |
acetanilide | substrate 4-nitroacetanilide, competitive inhibition | Delftia acidovorans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
