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Literature summary for 3.5.1.5 extracted from
- Purification and properties of urease from Brevibacterium ammoniagenes (1984), Agric. Biol. Chem., 48, 1495-1502.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 39% loss of activity | Corynebacterium ammoniagenes |  |
| Cu2+ | 1 mM, complete inactivation | Corynebacterium ammoniagenes | |
| Fe2+ | 1 mM, 42% loss of activity | Corynebacterium ammoniagenes | |
| Hg2+ | 1 mM, complete inactivation | Corynebacterium ammoniagenes | |
| Hydroxyurea | - |
Corynebacterium ammoniagenes | |
| Ni2+ | 1 mM, 26% loss of activity | Corynebacterium ammoniagenes | |
| PCMB | 0.1 mM, complete loss of activity | Corynebacterium ammoniagenes | |
| Zn2+ | 1 mM, 36% loss of activity | Corynebacterium ammoniagenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18 | 72 | Urea | value depends on pH and type of buffer | Corynebacterium ammoniagenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Nickel | contains 0.8 gatom of nickel per 67000 g of protein | Corynebacterium ammoniagenes | |
| Nickel | nickel metalloenzyme | Corynebacterium ammoniagenes | |
| Nickel | contains 1 atom of nickel per subunit | Corynebacterium ammoniagenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, SDS-PAGE | Corynebacterium ammoniagenes |
| 67000 | - |
3 * 67000, SDS-PAGE | Corynebacterium ammoniagenes |
| 215000 | - |
gel filtration | Corynebacterium ammoniagenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium ammoniagenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium ammoniagenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3500 | - |
- |
Corynebacterium ammoniagenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, 50 mM phosphate buffer, pH 7.5, 50% glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, about 30% loss of activity after 2 months | Corynebacterium ammoniagenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Corynebacterium ammoniagenes | CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, SDS-PAGE | Corynebacterium ammoniagenes |
| trimer | 3 * 67000, SDS-PAGE | Corynebacterium ammoniagenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | - |
Corynebacterium ammoniagenes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 40% of maximal activity, 70°C: about 10% of maximal activity | Corynebacterium ammoniagenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.5, 10 min, stable below | Corynebacterium ammoniagenes |
| 60 | - |
pH 7.5, 10 min, 50% loss of activity | Corynebacterium ammoniagenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Corynebacterium ammoniagenes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | 30°C, 5 h, stable | Corynebacterium ammoniagenes |
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