Literature summary for 3.5.1.5 extracted from

Reddy, K.R.; Kayastha, A.M.
Improved stability of urease upon coupling to alkylamine and arylamine glass and its analytical use (2006), J. Mol. Catal. B, 38, 104-112.

No PubMed abstract available

General Stability

General Stability	Organism
the immobilized enzyme is more stable in wet condition than in semi-dry condition and storing it at 4°C is more favourable over 27°C. There is a tremendous decrease in activity when the immobilized urease is not stored in buffer (i.e. semi-dry condition) and storing it at 4°C improves the longevity. Both the alkyl and arylamine-urease in wet condition stored at 4°C are further reused (30times) and the residual activity are 40% and 20%, respectively	Cajanus cajan

Organism

the immobilized enzyme is more stable in wet condition than in semi-dry condition and storing it at 4°C is more favourable over 27°C. There is a tremendous decrease in activity when the immobilized urease is not stored in buffer (i.e. semi-dry condition) and storing it at 4°C improves the longevity. Both the alkyl and arylamine-urease in wet condition stored at 4°C are further reused (30times) and the residual activity are 40% and 20%, respectively

Cajanus cajan

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
3.35	-	Urea	soluble enzyme	Cajanus cajan
3.43	-	Urea	arylamine glass-immobilized urease	Cajanus cajan
3.55	-	Urea	alkylamine glass-immobilized urease	Cajanus cajan

Organism

Organism	UniProt	Comment	Textmining
Cajanus cajan	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Cajanus cajan

Storage Stability

Storage Stability	Organism
4°C, 70 days, soluble enzyme loses 90% of its activity, alkylamine-immobilized enzyme loses 15% of the initial activity, the arylamine-immobilized enzyme loses 17% of its activity	Cajanus cajan

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
urea + H2O	-	Cajanus cajan	CO2 + NH3	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
47	-	soluble enzyme	Cajanus cajan
77	-	urease coupled to the alkylamine glass or arylamine glass	Cajanus cajan

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
77	-	15 min, soluble urease has a residual activity of 22%, alkylamine glass-immobilized enzyme and arylamine glass-immobilized urease retain 96% and 98%, respectively. Even after 90 min of incubation immobilized urease retains 50% of activity	Cajanus cajan

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	alkylamine glass-immobilized enzyme	Cajanus cajan
7	-	arylamine glass-immobilized enzyme	Cajanus cajan
7.3	-	soluble enzyme	Cajanus cajan

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3.5	-	22°C, 12 h, soluble enzyme retains 5% of its original activity, alkylamine glass-immobilized enzyme and arylamine glass immobilized urease retain 37% and 38%, respectively	Cajanus cajan